跨越骨骼肌三联体连接间隙的桥接结构。
Bridging structures spanning the junctioning gap at the triad of skeletal muscle.
作者信息
Somlyo A V
出版信息
J Cell Biol. 1979 Mar;80(3):743-50. doi: 10.1083/jcb.80.3.743.
Abstract
The membrane systems of skeletal muscle were examined after tannic acid fixation. A new structure consisting of bridges spanning the junctional gap is described, and a model is proposed in which the cytoplasmic but not the luminal membrane leaflets of the transverse tubule and of the junctional sarcoplasmic reticulum (SR) are continuous. The globular particles (presumably the Ca-binding proteins) within the terminal cisternae were arranged in longitudinal rows and appeared adherent to the junctional membrane. The junctional gap was present in negatively stained, frozen thin sections of fixed muscles. Negatively staining material occured within the junctional gap. The cytoplasmic leaflets of the longitudinal, intermediate, and terminal cisterna regions of the SR exhibited a thick coat of densely staining material compatible with the presence of the Ca-ATPase. Similar bridges were also observed at the surface membrane-SR close coupling sites of vascular smooth muscle.
摘要
在经单宁酸固定后，对骨骼肌的膜系统进行了检查。描述了一种由跨越连接间隙的桥组成的新结构，并提出了一个模型，其中横小管和连接肌浆网（SR）的胞质而非管腔膜小叶是连续的。终末池内的球状颗粒（可能是钙结合蛋白）呈纵向排列，并似乎附着于连接膜上。在固定肌肉的负染冷冻薄切片中存在连接间隙。在连接间隙内出现了负染物质。SR的纵向、中间和终末池区域的胞质小叶表现出一层厚厚的密集染色物质，这与钙ATP酶的存在相符。在血管平滑肌的表面膜 - SR紧密偶联位点也观察到了类似的桥。
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本文引用的文献
1
Studies on the endoplasmic reticulum. III. Its form and distribution in striated muscle cells.内质网的研究。III. 其在横纹肌细胞中的形态与分布。
J Biophys Biochem Cytol. 1957 Mar 25;3(2):269-300. doi: 10.1083/jcb.3.2.269.
2
A strand of cardiac muscle. Its ultrastructure and the electrophysiological implications of its geometry.一束心肌。其超微结构及其几何形状的电生理意义。
J Cell Biol. 1967 Apr;33(1):103-29. doi: 10.1083/jcb.33.1.103.
3
Inward spread of activation in vertebrate muscle fibres.脊椎动物肌纤维中激活的内向传播。
J Physiol. 1971 Feb;212(3):777-99. doi: 10.1113/jphysiol.1971.sp009356.
4
The fine structure of skeletal muscle triad junctions.骨骼肌三联体连接的精细结构。
J Ultrastruct Res. 1969 Oct;29(1):37-49. doi: 10.1016/s0022-5320(69)80054-7.
5
Membrane-to-membrane cross-bridges. A means to orientation and interaction of membrane faces.膜间交叉桥。一种使膜面定向和相互作用的方式。
J Cell Biol. 1971 Dec;51(3):881-8. doi: 10.1083/jcb.51.3.881.
6
7
Charge movement in the membrane of striated muscle.横纹肌细胞膜中的电荷移动。
J Physiol. 1976 Jan;254(2):339-60. doi: 10.1113/jphysiol.1976.sp011235.
8
The probable role of phosphatidyl cholines in the tannic acid enhancement of cytomembrane electron contrast.磷脂酰胆碱在鞣酸增强细胞膜电子对比度中可能发挥的作用。
J Cell Biol. 1977 Sep;74(3):742-6. doi: 10.1083/jcb.74.3.742.
9
Composition of sarcoplasmic reticulum in situ by electron probe X-ray microanalysis.
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10
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