Tanaka M, Nishikimi M, Suzuki H, Ozawa T, Ichiki T, Kobayashi M, Wada Y
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Biochem Int. 1987 Apr;14(4):735-9.
Enzymic activity and the levels of immunochemically detectable subunits of NADH-ubiquinone oxidoreductase (Complex I) were measured in the mitochondria from various tissues of a patient with mitochondrial encephalomyopathy and renal dysfunction. Rotenone-sensitive NADH-cytochrome c reductase activity was decreased in all the tissues examined, but the degree of deficiency varied from tissue to tissue. The levels of subunits in Complex I were decreased roughly in parallel with the activity in each tissue. These results indicate that the apparently tissue-specific manifestation of symptoms depends mainly on the levels of subunits in Complex I.
在一名患有线粒体脑肌病和肾功能障碍患者的各种组织的线粒体中，测定了NADH-泛醌氧化还原酶（复合体I）的酶活性和免疫化学可检测亚基的水平。在所检查的所有组织中，鱼藤酮敏感的NADH-细胞色素c还原酶活性均降低，但缺乏程度因组织而异。复合体I中亚基的水平大致与每个组织中的活性平行降低。这些结果表明，症状明显的组织特异性表现主要取决于复合体I中亚基的水平。
