Bride Lais de Lima, Pereira Monalessa Fábia, Barbosa Maralisi Coutinho, Silva Nayara Carvalho, Klein Nazareth Magnago, Nascimento Thiago César, Schuenck Ricardo Pinto
Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
Hospital Universitário Cassiano Antônio de Moraes (HUCAM), Vitória, ES, Brasil.
Rev Soc Bras Med Trop. 2019 Jul 18;52:e20190095. doi: 10.1590/0037-8682-0095-2019.
Staphylococcus aureus is a major nosocomial pathogen that is associated with high virulence and the rapid development of drug resistance.
We analyzed and compared the antimicrobial resistance, virulence profiles, and molecular epidemiology of 67 S. aureus strains, including 36 methicillin-sensitive (MSSA) and 31 methicillin-resistant (MRSA) strains recovered from a public hospital located in south-eastern Brazil.
The clones circulating in this hospital presented a great diversity, and the majority of the strains were related to clones responsible for causing worldwide epidemics: these included USA100 (New York/Japan clone), USA300, and USA600. The 31 MRSA (22 SCCmecII and 9 SCCmecIV) and 36 MSSA strains exhibited low resistance against gentamicin and trimethoprim/sulfamethoxazole. No MRSA strain showed resistance to tetracycline. Virulence gene carriage was more diverse and abundant in MSSA than in MRSA. Of the evaluated adhesion-related genes, ebpS was the most prevalent in both MSSA and MRSA strains. The genes bbp and cna showed a strong association with MSSA strains.
Our findings reinforce the idea that MSSA and MRSA strains should be carefully monitored, owing to their high pathogenic potential.
金黄色葡萄球菌是一种主要的医院病原体，具有高毒力和耐药性快速发展的特点。
我们分析并比较了67株金黄色葡萄球菌的耐药性、毒力特征和分子流行病学，其中包括从巴西东南部一家公立医院分离出的36株甲氧西林敏感（MSSA）菌株和31株甲氧西林耐药（MRSA）菌株。
该医院流行的克隆呈现出高度多样性，大多数菌株与导致全球流行的克隆有关：包括USA100（纽约/日本克隆）、USA300和USA600。31株MRSA（22株SCCmecII和9株SCCmecIV）和36株MSSA菌株对庆大霉素和甲氧苄啶/磺胺甲恶唑的耐药性较低。没有MRSA菌株对四环素耐药。MSSA菌株中与毒力相关基因的携带情况比MRSA菌株更多样化且更丰富。在评估的与黏附相关的基因中，ebpS在MSSA和MRSA菌株中最为普遍。bbp和cna基因与MSSA菌株有很强的关联性。
我们的研究结果强化了这样一种观点，即由于MSSA和MRSA菌株具有很高的致病潜力，因此应仔细监测它们。
