Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Department of Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Sci Rep. 2021 Mar 1;11(1):4228. doi: 10.1038/s41598-021-83661-8.
Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host's protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently associated with serious infections in human. We aim to investigate the genomic features of a highly virulent yet pan susceptible MSSA strain (coded as HS-MSSA) which caused concurrent bacteraemia in a dengue patient, ultimately resulted in sepsis death of the patient. Whole genome sequence analysis was performed. The draft genome of HS-MSSA is approximately 2.78 Mb (GC content = 32.7%) comprising of 2637 predicted coding sequences. In silico genotyping of the HS-MSSA strain revealed a novel combined genotype (t091/ST2990). The HS-MSSA carries a SaPIn1-like pathogenicity island that harbours the staphylococcal enterotoxin and enterotoxin-like genes (sec3 and selL). The strain-specific β-lactamase (blaZ)-bearing plasmid region was identified in HS-MSSA. Core genome phylogeny showed that the HS-MSSA strain shared a common ancestry with the European MRSA clone. We report herein the genomic features of an MSSA lineage with novel genotype previously not reported elsewhere.
金黄色葡萄球菌（S. aureus）是一种机会性病原体，一旦侵入宿主的保护屏障，就有可能对易感个体造成严重的健康影响。耐甲氧西林金黄色葡萄球菌（MSSA）虽然常与人类的严重感染有关，但往往受到较少关注。我们旨在研究一株高毒力但普遍敏感的 MSSA 菌株（编码为 HS-MSSA）的基因组特征，该菌株导致登革热患者同时发生菌血症，最终导致患者败血症死亡。进行了全基因组序列分析。HS-MSSA 的草图基因组约为 2.78 Mb（GC 含量=32.7%），包含 2637 个预测编码序列。HS-MSSA 的计算机基因分型显示了一种新型的组合基因型（t091/ST2990）。HS-MSSA 携带 SaPIn1 样致病性岛，其中含有葡萄球菌肠毒素和肠毒素样基因（sec3 和 selL）。在 HS-MSSA 中鉴定出了携带 blaZ 的菌株特异性β-内酰胺酶（blaZ）质粒区域。核心基因组系统发育分析表明，HS-MSSA 菌株与欧洲 MRSA 克隆具有共同的祖先。我们在此报告了一种以前在其他地方未报道过的新型基因型 MSSA 谱系的基因组特征。
