检测菲律宾马尼拉大都市区野外采集的埃及伊蚊中的沃尔巴克氏体。
Detection of Wolbachia in field-collected Aedes aegypti mosquitoes in metropolitan Manila, Philippines.
机构信息
Department of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan.
Biology Department, De La Salle University, Taft Avenue, Manila, Philippines.
出版信息
Parasit Vectors. 2019 Jul 24;12(1):361. doi: 10.1186/s13071-019-3629-y.
BACKGROUND
Recent reports reveal the presence of Wolbachia in Ae. aegypti. Our study presents additional support for Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using two Wolbachia-specific molecular makers.
METHODS
A total of 672 Ae. aegypti adult mosquitoes were collected from May 2014 to January 2015 in Metropolitan Manila. Each individual sample was processed and screened for the presence of Wolbachia by selected markers, Wolbachia-specific 16S rDNA and its surface protein (wsp), under optimized PCR conditions and sequenced.
RESULTS
Totals of 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A and B while the majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples showed positive amplifications in both markers and 69% showed congruence in supergroup identification (supergroup B).
CONCLUSIONS
By utilizing two Wolbachia-specific molecular makers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low Wolbachia infection rate and inferred the detected strains belong to either supergroups A and B.
背景
最近的报告显示，埃及伊蚊中存在沃尔巴克氏体。我们的研究通过使用两种沃尔巴克氏体特异性分子标记物筛选野外采集的成年蚊子，为埃及伊蚊中沃尔巴克氏体感染提供了额外的支持。
方法
从 2014 年 5 月至 2015 年 1 月，在马尼拉大都会共收集了 672 只埃及伊蚊成年蚊子。每个个体样本都经过处理，并通过选择的标记物（沃尔巴克氏体特异性 16S rDNA 和其表面蛋白（wsp））在优化的 PCR 条件下进行筛选，以确定是否存在沃尔巴克氏体，并进行测序。
结果
使用 wsp 和 16S rDNA 标记物，分别有 113 只（16.8%）和 89 只（13.2%）个体蚊子样本被确定感染了沃尔巴克氏体。埃及伊蚊 wsp 样本序列与属于超级群 A 和 B 的五种已知沃尔巴克氏体菌株相似或相同，而大多数 16S rDNA 样本序列与属于超级群 B 的菌株相似。总体而言，80 只（11.90%）个体蚊子样本在两个标记物中均显示阳性扩增，69%的样本在超级群鉴定上具有一致性（超级群 B）。
结论
通过使用两种沃尔巴克氏体特异性分子标记物，我们的研究从单个埃及伊蚊样本中证实了沃尔巴克氏体的存在。我们的结果显示沃尔巴克氏体感染率较低，并推断检测到的菌株属于超级群 A 和 B。
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