Department of Biology, Marmara University, Göztepe Campus, Kadıköy, 34722, Istanbul, Turkey.
Protoplasma. 2020 Jan;257(1):89-101. doi: 10.1007/s00709-019-01422-x. Epub 2019 Jul 24.
The aim of the current study was to examine the effect of different exogenous putrescine concentrations (200, 400, 600, and 800 μM) on the tea pollen performance. It was shown that putrescine has a dose-dependent effect on pollen performance. Results exhibited that pollen germination and tube elongation were induced by 200 and 400 μM putrescine treatment, especially, 400 μM putrescine-enhanced pollen performance. However, pollen performance was inhibited by higher concentrations of putrescine. Putrescine concentrations above 400 μM changed the actin filament distribution in pollen tubes by affecting the distribution of sucrose synthase enzyme. Alterations of the distribution on sucrose synthase enzyme also caused the alterations in the dispersion of cellulose and callose in the cell wall, and morphological alterations such as balloon-shaped and snake-shaped pollen tube tip accompanied them. Moreover, putrescine concentrations above 400 μM caused a decrease of ROS level in apex and led to chromatin condensation of the generative nucleus. In conclusion, exogenous putrescine application can be used as a pollen performance enhancer at low concentrations while the high concentrations cause adverse effects reducing fertilization success.
本研究旨在探讨不同外源腐胺浓度（200、400、600 和 800 μM）对茶花粉性能的影响。结果表明，腐胺对花粉性能具有剂量依赖性影响。结果表明，200 和 400 μM 腐胺处理诱导花粉萌发和花粉管伸长，特别是 400 μM 腐胺增强花粉性能。然而，较高浓度的腐胺抑制花粉性能。腐胺浓度高于 400 μM 会通过影响蔗糖合酶酶的分布来改变花粉管中的肌动蛋白丝分布。蔗糖合酶酶分布的改变也导致细胞壁中纤维素和胼胝质的分散发生改变，伴随着形态学改变，如气球状和蛇形花粉管尖端。此外，腐胺浓度高于 400 μM 会导致顶端 ROS 水平降低，并导致生殖核染色质浓缩。总之，外源腐胺的应用可以在低浓度下作为花粉性能增强剂，而高浓度会降低受精成功率，产生不良影响。
