经乙二胺四乙酸（EDTA）溶液预处理的大肠杆菌细胞的电刺激转化

Electro-stimulated transformation of E. coli cells pretreated by EDTA solution.

作者信息

Cymbalyuk E S, Chernomordik L V, Broude N E

机构信息

A.N. Frumkin Institute of Electrochemistry, USSR Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1988 Jul 4;234(1):203-7. doi: 10.1016/0014-5793(88)81334-6.

DOI:10.1016/0014-5793(88)81334-6

PMID:3134254

Abstract

The phenomenon of transformation of E. coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.5 ms) to the suspension. The maximum transformation efficiency obtained was 4 X 10(6) colonies/micrograms DNA. The maximum transformation frequency was 0.4% at a DNA concentration of 15 micrograms/ml.

摘要

对大肠杆菌细胞在电处理下的转化现象进行了研究。在等渗培养基中经乙二胺四乙酸（EDTA）处理后的MH 1、HB 101和DH 1菌株的细胞，通过对悬浮液施加单个指数脉冲（E = 10 kV/cm，T = 1.5 ms）用pBR322 DNA进行转化。获得的最大转化效率为4×10⁶个菌落/微克DNA。在DNA浓度为15微克/毫升时，最大转化频率为0.4%。