Tang Rong, She Qinying, Lu Yinghui, Yin Ru, Zhu Ping, Zhu Ling, Zhou Minlin, Zheng Chunxia
National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
Biopreserv Biobank. 2019 Oct;17(5):477-482. doi: 10.1089/bio.2019.0029. Epub 2019 Jul 25.
The PAXgene Blood RNA tube is used to protect RNA of whole blood, and the stability of RNA in this tube has already been reported. However, there are few reports on the quality of RNA from long-term preservation of these tubes. Our biobank conducted quality control of RNA extracted from the tubes after varying storage periods. A total of 300 blood samples of renal disease patients were randomly selected at each time point (from 1 month to 7 years). Median RNA yields were 3.46 (2.65-4.87) μg, 4.34 (3.20-5.87) μg, 4.77 (2.88-6.29) μg, 4.19 (2.65-6.26) μg, 3.85 (2.43-6.13) μg, and 3.21 (1.85-6.61) μg at 1 month, 1, 2, 3, 6, and 7 years, respectively. There were no significant differences in RNA yields among all the storage periods. A ratios were 2.02 ± 0.04, 2.05 ± 0.04, 2.05 ± 0.04, 2.08 ± 0.03, 2.12 ± 0.10, and 2.11 ± 0.05, all of which were ≥1.8. However, A of the samples stored for 6 and 7 years had a rising trend, compared with the other time points ( < 0.05). Median RNA integrity number (RIN) values were 8.4 (7.6-9.1), 8.3 (7.7-8.9), 8.3 (7.8-8.8), 8.5 (8.3-8.9), 7.6 (7.1-8.1), and 7.9 (7.2-8.3) at each time point. Lower RIN values were found at 6 and 7 years compared with the other storage periods ( < 0.05). The rates of RIN values ≥7.0 were 92%, 84%, 96%, 92%, 86%, and 84%, which exhibited no differences across all the storage periods. In addition, the yields and RIN values of RNA from samples with blood clots were significantly lower than those without ( < 0.001). The quantity and quality of RNA extracted from PAXgene Blood RNA tubes are stable throughout cryopreservation for 7 years.
PAXgene血液RNA管用于保护全血RNA，该管中RNA的稳定性已有报道。然而，关于这些管子长期保存后的RNA质量报道较少。我们的生物样本库对不同保存期后从管子中提取的RNA进行了质量控制。在每个时间点（从1个月到7年）随机选取300例肾病患者的血液样本。1个月、1年、2年、3年、6年和7年时RNA产量中位数分别为3.46（2.65 - 4.87）μg、4.34（3.20 - 5.87）μg、4.77（2.88 - 6.29）μg、4.19（2.65 - 6.26）μg、3.85（2.43 - 6.13）μg和3.21（1.85 - 6.61）μg。所有保存期之间RNA产量无显著差异。A比率分别为2.02±0.04、2.05±0.04、2.05±0.04、2.08±0.03、2.12±0.10和2.11±0.05，均≥1.8。然而，与其他时间点相比，保存了6年和7年的样本的A有上升趋势（P<0.05）。每个时间点RNA完整性数（RIN）值中位数分别为8.4（7.6 - 9.1）、8.3（7.7 - 8.9）、8.3（7.8 - 8.8）、8.5（8.3 - 8.9）、7.6（7.1 - 8.1）和7.9（7.2 - 8.3）。与其他保存期相比，6年和7年时RIN值较低（P<0.05）。RIN值≥7.0的比率分别为92%、84%、96%、92%、86%和84%，在所有保存期之间无差异。此外，有血凝块样本的RNA产量和RIN值显著低于无血凝块样本（P<0.001）。从PAXgene血液RNA管中提取的RNA的数量和质量在7年的冷冻保存期内是稳定的。
