Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Department of Environmental Science, Dalhousie University, Halifax, Nova Scotia, Canada.
Bioresour Technol. 2019 Nov;291:121811. doi: 10.1016/j.biortech.2019.121811. Epub 2019 Jul 13.
Degradation of hydrolyzed polyacrylamide-containing (HPAM-containing) wastewater was investigated in a lab-scale aerobic-ozonic-aerobic hybrid treatment system. When the HPAM concentration was 500 mg L and the ozone dose was 25 g O/g TOC, the HPAM removal rate reached 90.79%. Experimental results obtained from gel permeation chromatography (GPC) and rheometer indicated that the refractory HPAM was decomposed into small-molecule compounds. High performance liquid chromatography (HPLC) analysis showed that there was no acrylamide (AM) in the effluent of the system. Microbial communities in two aerobic biofilm reactors (ABRs) were analyzed by Illumina MiSeq Sequencing, which indicated that norank_f_Cytophagaceae, Meiothermus, Bacillus, etc. were keystone functional bacterial genera and Methanobacterium, norank_p_Bathyarchaeota, norank_c_Marine_Group_Ⅰ, etc. were dominant functional archaeal groups. To our knowledge, this is the first study to treat HPAM-containing wastewater using an aerobic-ozonic-aerobic hybrid process. Good removal efficiencies and presence of functional microorganisms demonstrated that the hybrid treatment system was practical for treating HPAM-containing wastewater.
在实验室规模的好氧-臭氧化-好氧混合处理系统中研究了含水解聚丙烯酰胺（HPAM）废水的降解。当 HPAM 浓度为 500mg/L 且臭氧剂量为 25g O/g TOC 时，HPAM 的去除率达到 90.79%。凝胶渗透色谱（GPC）和流变仪的实验结果表明，难降解的 HPAM 被分解成小分子化合物。高效液相色谱（HPLC）分析表明系统出水中没有丙烯酰胺（AM）。通过 Illumina MiSeq 测序分析两个好氧生物膜反应器（ABR）中的微生物群落，结果表明，未分类的_f_Cytophagaceae、梅奥图姆斯、芽孢杆菌等是关键功能细菌属，甲烷杆菌、未分类的_p_Bathyarchaeota、未分类的_c_Marine_Group_Ⅰ等是主要功能古菌属。据我们所知，这是首次使用好氧-臭氧化-好氧混合工艺处理含 HPAM 废水的研究。良好的去除效率和功能微生物的存在证明了混合处理系统在处理含 HPAM 废水中的实用性。
