Ahmed S M, Kimura M, Itokawa Y
Department of Hygiene, Faculty of Medicine, Kyoto University, Japan.
J Am Coll Nutr. 1988 Jun;7(3):215-21. doi: 10.1080/07315724.1988.10720238.
To clarify the influence of protein calorie malnutrition (PCM) on thiamin metabolism in rats, the effects of three diets containing different amounts of protein and sucrose were evaluated by three tests in 20 young male Wistar rats equally divided into four groups. Three of the groups, the controls (group 1), those fed high protein (group 2), and one of the two groups fed a low protein diet (group 3), were fed ad libitum. A second group (group 4) on the same low protein diet was fed 40% less than that consumed by group 3, which consumed less than the controls or high protein rats because of poor appetite. The sucrose contents of the experimental diets were inversely related to the protein contents. Thiamin concentrations were determined in blood, brain, heart, liver, kidney and spleen, and in 24-hr urine and stools. Tissue transketolase activity (TTKA) was measured in brain and liver and in erythrocytes (ETKA), the latter in association with the thiamin pyrophosphate (TPP) effect. Malnutrition caused decreased 24-hr urinary thiamin excretion, decreased ETKA, with corresponding increased TPP effect and decreased TKA of brain. As tissue thiamin concentrations were not decreased, the results suggest that malnutrition causes functional thiamin deficiency.
为阐明蛋白质热量营养不良（PCM）对大鼠硫胺素代谢的影响，在20只雄性Wistar幼鼠中进行了三项试验，这些大鼠被平均分为四组，分别喂食三种含不同蛋白质和蔗糖量的日粮。其中三组，即对照组（第1组）、高蛋白日粮组（第2组）以及低蛋白日粮组中的一组（第3组），自由采食。另一组（第4组）同样喂食低蛋白日粮，但喂食量比第3组少40%，第3组因食欲不佳，其食量少于对照组或高蛋白日粮组大鼠。实验日粮中的蔗糖含量与蛋白质含量呈负相关。测定了血液、脑、心脏、肝脏、肾脏和脾脏、24小时尿液及粪便中的硫胺素浓度。测定了脑、肝脏和红细胞中的组织转酮醇酶活性（TTKA），红细胞中的转酮醇酶活性（ETKA）与硫胺素焦磷酸（TPP）效应相关。营养不良导致24小时尿硫胺素排泄减少、ETKA降低，相应地TPP效应增加，脑TKA降低。由于组织硫胺素浓度未降低，结果表明营养不良导致功能性硫胺素缺乏。
