异硫氰酸荧光素（FITC）单克隆抗体的制备及其放射免疫分析应用
The production and radioimmunoassay application of monoclonal antibodies to fluorescein isothiocyanate (FITC).
作者信息
Micheel B, Jantscheff P, Böttger V, Scharte G, Kaiser G, Stolley P, Karawajew L
机构信息
Central Institute of Molecular Biology, Academy of Sciences of the G.D.R., Berlin-Buch.
出版信息
J Immunol Methods. 1988 Jun 28;111(1):89-94. doi: 10.1016/0022-1759(88)90063-4.
PMID:3134489
Abstract
Monoclonal antibodies (MoAbs) were produced against the fluorescence marker fluorescein isothiocyanate (FITC). FITC was used as a hapten to label different proteins and the anti-FITC MoAbs were used to identify these labelled proteins in a solid-phase radioimmunoassay and in cellular radioimmuno-binding assays for the demonstration of antigens and antibodies.
摘要
制备了针对荧光标记物异硫氰酸荧光素（FITC）的单克隆抗体（MoAb）。FITC用作半抗原标记不同蛋白质，抗FITC单克隆抗体用于在固相放射免疫测定和细胞放射免疫结合测定中鉴定这些标记蛋白质，以证明抗原和抗体。
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