College of Pharmacy, Southwest Minzu University, Chengdu 610041, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Molecules. 2019 Jul 24;24(15):2699. doi: 10.3390/molecules24152699.
The bamboo shoot of (Keng) Keng f. is a medicinal and edible plant product in China. In this study, the chemical composition of the total alkaloids from bamboo shoots and bamboo shoot shells of (Keng) Keng f. (ABSP and ABSSP, respectively) were separated and investigated by UHPLC/QTOF-MS/MS. The results showed that a total of 32 alkaloids were extracted, with 15 common to both ABSP and ABSSP and 10 and 7 alkaloids distinct to ABSP and ABSSP, respectively. ABSP and ABSSP both decreased the lipopolysaccharide (LPS, 0.5 μg/mL)-induced nitric oxide (NO) production in RAW264.7 murine macrophages with half maximal inhibitory concentration (IC) values of 78 and 55 μg/mL, respectively. We also found that ABSP and ABSSP (100 μg/mL) could decrease the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both mRNA and protein levels in LPS-exposed RAW264.7 cells. Moreover, 100 μg/mL of ABSP and ABSSP also significantly inhibited LPS-induced mRNA expression of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). Additionally, ABSP and ABSSP (100 μg/mL) decreased the phosphorylation of extracellular regulated protein kinase (ERK) in LPS-stimulated RAW264.7 cells. Collectively, the total alkaloids from the bamboo shoots and shells of exhibit anti-inflammatory effects in LPS-activated RAW264.7 cells through the inhibition of ERK signaling. This result can provide support for the medicinal use and further study of .
（苦）竹的竹笋是中国的一种药食两用植物产品。在本研究中，采用 UHPLC/QTOF-MS/MS 对（苦）竹竹笋和竹壳总生物碱（ABSP 和 ABSSP）的化学成分进行了分离和研究。结果表明，共提取出 32 种生物碱，其中 15 种在 ABSP 和 ABSSP 中共有，10 种和 7 种生物碱分别在 ABSP 和 ABSSP 中特有。ABSP 和 ABSSP 均能降低脂多糖（LPS，0.5μg/mL）诱导的 RAW264.7 小鼠巨噬细胞一氧化氮（NO）的产生，半数最大抑制浓度（IC）值分别为 78 和 55μg/mL。我们还发现，ABSP 和 ABSSP（100μg/mL）可降低 LPS 暴露的 RAW264.7 细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达，无论是在 mRNA 水平还是蛋白水平。此外，100μg/mL 的 ABSP 和 ABSSP 还能显著抑制 LPS 诱导的白细胞介素 1β（IL-1β）和肿瘤坏死因子 α（TNF-α）的 mRNA 表达。此外，ABSP 和 ABSSP（100μg/mL）还降低了 LPS 刺激的 RAW264.7 细胞中细胞外调节蛋白激酶（ERK）的磷酸化。综上所述，（苦）竹的竹笋和竹壳总生物碱在 LPS 激活的 RAW264.7 细胞中通过抑制 ERK 信号通路发挥抗炎作用。该结果可为（苦）竹的药用价值和进一步研究提供支持。
