与大鼠脑中游离信使核糖核蛋白颗粒相关的细胞质多聚（ADP - 核糖）聚合酶

Cytoplasmic poly(ADP-ribose) polymerase associated with free messenger ribonucleoprotein particles in rat brain.

作者信息

Chypre C, Maniez C, Mandel P

机构信息

Centre de Neurochimie du CNRS, Strasbourg, France.

出版信息

J Neurochem. 1988 Aug;51(2):561-5. doi: 10.1111/j.1471-4159.1988.tb01075.x.

DOI:10.1111/j.1471-4159.1988.tb01075.x

PMID:3134510

Abstract

Poly(ADP-ribose) polymerase associated with free cytoplasmic messenger ribonucleoprotein particles (free mRNP particles) carrying messenger RNA has been characterized in rat brain. There were first-order kinetics for NAD with an apparent Km for NAD of 90.5 +/- 0.70 microM and Vmax of 19.7 +/- 2.8 pmol ADP-ribose incorporated min-1 mg protein-1. Five poly(ADP-ribose) protein acceptors were identified in the Mr 37,000-120,000 range. It is hypothesized that ADP-ribosylation of specific free mRNP proteins might play a role in the derepression and translation of the silent mRNAs of free mRNP particles.

摘要

与携带信使核糖核酸的游离细胞质信使核糖核蛋白颗粒（游离mRNP颗粒）相关的聚（ADP - 核糖）聚合酶已在大鼠脑中得到表征。NAD呈现一级动力学，NAD的表观Km为90.5±0.70微摩尔，Vmax为19.7±2.8皮摩尔ADP - 核糖掺入每分钟每毫克蛋白质。在37,000 - 120,000道尔顿范围内鉴定出了五种聚（ADP - 核糖）蛋白质受体。据推测，特定游离mRNP蛋白质的ADP - 核糖基化可能在游离mRNP颗粒沉默mRNA的去抑制和翻译中起作用。