Zoology department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-0193, Japan.
J Trace Elem Med Biol. 2019 Sep;55:82-88. doi: 10.1016/j.jtemb.2019.06.007. Epub 2019 Jun 14.
Exposure to the environmental pollutants poses a serious threat to aquatic organism. The arsenic exposure in fish increases the risk of developing serious alterations from embryo to adult.
The present investigation was done to study the toxic effects of heavy metal arsenic [As(III)] on medaka (Oryzias latipes). Morphological alterations, apoptosis, nuclear abnormalities, and genotoxic biomarkers in erythrocytes were used to determine the stress caused by arsenic (As) exposure.
Medaka was exposed to As for 15 days at two toxic sublethal concentrations (7 ppm and 10 ppm) in combination with Spirulina platensis (SP) treatment as antioxidant algae at 200 mg/L.
Results were consistent with a previous study results on tilapia. Exposure of medaka to As resulted in a dose-dependent increase in most the biomarkers used in the current study. Fish exposed to10 ppm As showed highest level of DNA damage. For the first time to our knowledge, using SP to counter the As toxicity in medaka, DNA damage restored to control levels.
Accordingly, those results suggests that SP can protect medaka in aquaculture against As-induced damage by its ability as reactive oxygen species (ROS) reducer, antioxidant role, and DNA damage scavenger.
暴露于环境污染物对水生生物构成严重威胁。鱼类暴露于砷会增加从胚胎到成年期发生严重变化的风险。
本研究旨在研究重金属砷 [As(III)] 对青鳉（Oryzias latipes）的毒性作用。使用形态改变、细胞凋亡、核异常和红细胞遗传毒性生物标志物来确定砷 (As) 暴露引起的应激。
在两种有毒亚致死浓度（7 ppm 和 10 ppm）下，将青鳉暴露于砷中 15 天，并与作为抗氧化藻类的螺旋藻（SP）处理（200 mg/L）相结合。
结果与先前关于罗非鱼的研究结果一致。青鳉暴露于砷中会导致当前研究中使用的大多数生物标志物呈剂量依赖性增加。暴露于 10 ppm As 的鱼类表现出最高水平的 DNA 损伤。据我们所知，首次使用 SP 来对抗青鳉中的砷毒性，DNA 损伤恢复到对照水平。
因此，这些结果表明 SP 可以通过其作为活性氧 (ROS) 还原剂、抗氧化作用和 DNA 损伤清除剂的能力，保护水产养殖中的青鳉免受砷诱导的损伤。
