Korbut A I, Klimontov V V, Orlov N B, Khotskina A S, Zav'yalov E L
Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2019 Jul;167(3):325-328. doi: 10.1007/s10517-019-04519-8. Epub 2019 Jul 26.
We studied the relationships between body composition parameters and plasma levels of pancreatic, gut, and adipose tissue hormones regulating energy balance and glucose metabolism in diabetic db/db mice (BKS.Cg-Dock7Lepr/J). The body composition parameters in mice aged 8, 12, and 16 weeks were assessed by magnetic resonance imaging. The concentrations of insulin, glucagon, ghrelin, glucagon-like peptide-1, glucose-dependent immunotropic peptide, leptin, resistin, and plasminogen activator-1 were measured by multiplex analysis at the age of 8 and 16 weeks. In comparison with non-diabetic control (db/+), db/db mice demonstrated high fat mass and reduced lean body mass and water content. In 8- and 16-week-old db/db mice, the levels of leptin (p<0.001), insulin (p<0.01), and glucagon-like peptide-1 (p<0.05) were elevated and the concentration of ghrelin (p<0.05) was reduced. The body weight and fat mass positively correlated with the levels of leptin, insulin, plasminogen activator-1, and glucagon-like peptide-1 and negatively correlated with ghrelin concentration. The results provide further details for characteristics of db/db mice, a widely used model of obesity and type 2 diabetes mellitus.
我们研究了糖尿病db/db小鼠（BKS.Cg-Dock7Lepr/J）的身体成分参数与调节能量平衡和葡萄糖代谢的胰腺、肠道及脂肪组织激素血浆水平之间的关系。通过磁共振成像评估8周、12周和16周龄小鼠的身体成分参数。在8周龄和16周龄时，采用多重分析法测定胰岛素、胰高血糖素、胃饥饿素、胰高血糖素样肽-1、葡萄糖依赖性促胰岛素多肽、瘦素、抵抗素和纤溶酶原激活物-1的浓度。与非糖尿病对照（db/+）相比，db/db小鼠表现出高脂肪量、低瘦体重和低含水量。在8周龄和16周龄的db/db小鼠中，瘦素水平（p<0.001）、胰岛素水平（p<0.01）和胰高血糖素样肽-1水平（p<0.05）升高，胃饥饿素浓度（p<0.05）降低。体重和脂肪量与瘦素、胰岛素、纤溶酶原激活物-1和胰高血糖素样肽-1水平呈正相关，与胃饥饿素浓度呈负相关。这些结果为db/db小鼠（一种广泛应用的肥胖和2型糖尿病模型）的特征提供了更多细节。
