Smagliy L V, Yartseva Yu O, Rydchenko V S, Birulina Yu G, Gusakova S V, Kovalev I V, Petrova I V, Nosarev A V
Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
Bull Exp Biol Med. 2019 Jul;167(3):363-366. doi: 10.1007/s10517-019-04527-8. Epub 2019 Jul 26.
We studied the role of carbon monoxide (CO) in the effect of P2X and P2Y receptor agonist ATP on the tone of rat aorta segments with intact endothelium. ATP (1-1000 μM) and P2X receptor agonist α,β-MeATP (100 μM) relaxed segments precontracted with phenylephrine (10 μM), while UTP (100-1000 μM) increased the amplitude of phenylephrine-induced contraction. The relaxing effect of ATP was enhanced by CORM II (100 μM), NO synthase inhibitor L-NAME, and guanylate cyclase inhibitor ODQ and attenuated by ZnPP IX (100 μM). The constrictive effect of UTP was weakened by CORM II (100 μM), but was not changed by ZnPP IX (100 μM). ZnPP IX (100 μM) weakened the relaxation response to α,β-MeATP. Thus, ATP involves the CO-dependent signaling cascade through P2X receptors.
我们研究了一氧化碳（CO）在P2X和P2Y受体激动剂ATP对具有完整内皮的大鼠主动脉段张力的影响中所起的作用。ATP（1 - 1000 μM）和P2X受体激动剂α,β - MeATP（100 μM）可使预先用去氧肾上腺素（10 μM）预收缩的血管段舒张，而UTP（100 - 1000 μM）则增加去氧肾上腺素诱导的收缩幅度。CORM II（100 μM）、一氧化氮合酶抑制剂L - NAME和鸟苷酸环化酶抑制剂ODQ可增强ATP的舒张作用，而ZnPP IX（100 μM）则使其减弱。CORM II（100 μM）可减弱UTP的收缩作用，但ZnPP IX（100 μM）对其无影响。ZnPP IX（100 μM）减弱了对α,β - MeATP的舒张反应。因此，ATP通过P2X受体参与了依赖CO的信号级联反应。
