Department of Chemistry, Natural Sciences Complex, University at Buffalo, Buffalo, NY, USA.
Adv Exp Med Biol. 2019;1140:111-119. doi: 10.1007/978-3-030-15950-4_6.
Proteomics-based mass spectrometry has gained increasing amounts of popularity in recent years. In particular, high resolution accurate mass measurements in mass spectrometry has gained notoriety for giving the capability of high throughput analysis with lower cost to the user. In particular, its uses in the identification of protein sequence through the utilization of bottom-up, middle-down, and top-down approaches has been widely discussed. In this chapter, we discuss the advantages of each technique as well as using the techniques in tandem to gain well-rounded structural data on our protein of interest, glucokinase. The study will focus on the use of Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry, but give insights into the advantages that may come from the utilization of other high resolution techniques.
基于蛋白质组学的质谱分析近年来越来越受到欢迎。特别是，质谱中高分辨率精确质量测量因其具有高通量分析的能力和更低的用户成本而受到关注。特别是，它在通过使用自上而下、中向和自下而上方法鉴定蛋白质序列方面的应用已经得到了广泛的讨论。在本章中，我们讨论了每种技术的优点，并讨论了串联使用这些技术以获得我们感兴趣的蛋白质葡萄糖激酶全面的结构数据。该研究将重点介绍傅里叶变换离子回旋共振（FT-ICR）质谱的使用，但也将探讨利用其他高分辨率技术可能带来的优势。
