Nakano Yosuke, Taniguchi Moyu, Umakoshi Yutaka, Watai Daisuke, Fukusaki Eiichiro
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
Methods Mol Biol. 2019;2030:253-261. doi: 10.1007/978-1-4939-9639-1_19.
D-Amino acids have recently attracted much attention in various research fields including medical, clinical, and food industry due to their important biological functions that differ from L-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 D-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.
由于D-氨基酸具有不同于L-氨基酸的重要生物学功能，它们最近在医学、临床和食品工业等各个研究领域引起了广泛关注。大多数手性氨基酸分离技术需要复杂的衍生化程序，以实现理想的色谱行为和可检测性。本章介绍了一种使用液相色谱-串联质谱（LC-MS/MS）在无需任何衍生化过程的情况下对手性氨基酸进行对映体分离的高灵敏度分析方法。该方法能够以高灵敏度和重现性同时分析18种D-氨基酸。此外，本章还重点介绍了该方法在实际样品中用于定量目标氨基酸的应用。
