Werneth Madeleine, Koellensperger Gunda, Hann Stephan
Department of Chemistry, University of Natural Resources and Life Sciences-BOKU Vienna, Vienna, Austria.
Methods Mol Biol. 2019;2030:395-402. doi: 10.1007/978-1-4939-9639-1_29.
Analysis of underivatized amino acids is challenging regarding both separation and detection of this small, polar, and largely UV-inactive compounds. Additives for reversed phase chromatography such as ion pairing reagents can hamper mass spectrometric detection. Zwitterionic hydrophilic interaction chromatography using MS compatible eluents together with tandem mass spectrometry in multiple reaction monitoring mode for selective detection is an attractive approach to overcome the abovementioned issues.
对于这些小分子、极性且大多无紫外活性的化合物，未衍生化氨基酸的分析在分离和检测方面都具有挑战性。反相色谱中的添加剂（如离子对试剂）会妨碍质谱检测。采用与质谱兼容的洗脱液的两性离子亲水相互作用色谱，结合多反应监测模式下的串联质谱进行选择性检测，是克服上述问题的一种有吸引力的方法。
