化脓性链球菌1型和3型M蛋白：胃蛋白酶提取及纤维蛋白原结合特性

Type 1 and 3 M-proteins of Streptococcus pyogenes: peptic extraction and fibrinogen binding properties.

作者信息

Kühnemund O, Moravek L, Havlicek J, Köhler W

机构信息

Academy of Sciences of the GDR, Central Institute of Microbiology and Experimental Therapy, Jena.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Mar;268(1):24-32. doi: 10.1016/s0176-6724(88)80110-x.

DOI:10.1016/s0176-6724(88)80110-x

PMID:3134767

Abstract

The pepsin extraction of group A type 1 streptococci for the isolation of M protein fragments was studied at different pH values and at different time intervals. The extracts were compared by SDS PAGE and fused rocket immunoelectrophoresis. Type 1 M protein fragments were prepared in preparative scale by pepsin extraction of type 1 streptococci at pH 5.5 for 60 min. The fragments were separated by affinity chromatography on immobilized fibrinogen and finally purified for sequence studies by gel chromatography. Pepsin extraction of group A type 3 streptococci was also studied at different pH values. In contrast to type 1, the SDS PAGE pattern changed drastically in dependence on the pH. Affinity chromatography on immobilized fibrinogen is also effective in the separation of the pH 5.5 type 3 streptococcal pepsin extract.

摘要

研究了在不同pH值和不同时间间隔下，用胃蛋白酶提取A组1型链球菌以分离M蛋白片段的情况。通过SDS-PAGE和融合火箭免疫电泳对提取物进行比较。通过在pH 5.5条件下用胃蛋白酶提取1型链球菌60分钟，以制备规模制备1型M蛋白片段。通过固定化纤维蛋白原的亲和色谱法分离片段，最后通过凝胶色谱法纯化以进行序列研究。还研究了在不同pH值下用胃蛋白酶提取A组3型链球菌的情况。与1型不同，SDS-PAGE图谱根据pH值有很大变化。固定化纤维蛋白原的亲和色谱法在分离pH 5.5的3型链球菌胃蛋白酶提取物中也有效。