Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine , China Agricultural University , Beijing 100193 , People's Republic of China.
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning , Beijing Center for Disease Prevention and Control , Beijing 100013 , People's Republic of China.
J Agric Food Chem. 2019 Aug 14;67(32):9088-9095. doi: 10.1021/acs.jafc.9b01757. Epub 2019 Aug 5.
Triclocarban (TCC) and triclosan (TCS) have been simultaneously detected in five kinds of foodstuffs using an immunoaffinity purification method coupled with ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for the first time. Two highly specific monoclonal antibodies against TCC and TCS were produced and coupled to -hydroxysuccinimide-activated Sepharose 6B gel to prepare the immunosorbent. Under the optimal conditions, mean recoveries from spiked samples by the IAC-UHPLC-MS/MS method were 70.1-92.8% for TCC and 76.6-102.5% for TCS. Intraday relative standard deviations were below 14.5%. The limits of quantification (LOQs) of TCC were 1 ng/L for beverage samples and 0.01-0.02 μg/kg for food samples. The LOQs of TCS were 0.03 μg/L for beverage samples and 0.2-0.3 μg/kg for food samples. The applicability of the method has been proven by analyzing TCC and TCS in different samples from supermarkets in Beijing. The proposed method is sufficiently sensitive and reliable for monitoring trace concentrations of TCC and TCS in food samples.
首次采用免疫亲和净化法结合超高效液相色谱-串联质谱法（UHPLC-MS/MS）同时检测了五种食品中的三氯卡班（TCC）和三氯生（TCS）。制备了两种针对 TCC 和 TCS 的高特异性单克隆抗体，并将其偶联到 - 羟基琥珀酰亚胺活化的 Sepharose 6B 凝胶上，制备了免疫吸附剂。在最佳条件下，IAC-UHPLC-MS/MS 方法从加标样品中的平均回收率为 TCC 的 70.1-92.8%，TCS 的 76.6-102.5%。日内相对标准偏差低于 14.5%。TCC 的定量限（LOQ）为饮料样品的 1 ng/L 和食品样品的 0.01-0.02 μg/kg。TCS 的 LOQ 为饮料样品的 0.03 μg/L 和食品样品的 0.2-0.3 μg/kg。该方法已通过分析北京超市不同样品中的 TCC 和 TCS 得到验证。该方法足够灵敏可靠，可用于监测食品样品中痕量 TCC 和 TCS 的浓度。
