Fey M F, Wainscoat J S
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Am J Clin Pathol. 1988 Aug;90(2):187-9. doi: 10.1093/ajcp/90.2.187.
DNA samples of two patients with acute myeloblastic leukemia were noted to have a variant band on digestion with HindIII and hybridization to a probe for the joining region of the immunoglobulin (Ig) heavy chain gene (JH). Further analysis showed that the variant band represented a constitutional DNA polymorphism rather than an Ig gene rearrangement. The HindIII fragment detected by the JH probe includes a highly polymorphic region 5' to the Ig heavy chain locus, and hence variant germline bands may be seen on autoradiography that can be mistaken for Ig gene rearrangements. The use of several different restriction enzymes and the analysis of the constitutional DNA allows a clear distinction to be made between somatic gene rearrangements and DNA polymorphisms.
两名急性髓细胞白血病患者的DNA样本在用HindIII消化并与免疫球蛋白（Ig）重链基因（JH）连接区探针杂交后，发现有一条变异带。进一步分析表明，该变异带代表一种组成型DNA多态性，而非Ig基因重排。JH探针检测到的HindIII片段包括Ig重链基因座5'端的一个高度多态性区域，因此在放射自显影片上可能会看到变异的种系带，这些带可能会被误认为是Ig基因重排。使用几种不同的限制性内切酶以及对组成型DNA进行分析，可以明确区分体细胞基因重排和DNA多态性。
