Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, Sichuan, China.
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610064, China.
Pathog Dis. 2019 Jun 1;77(4). doi: 10.1093/femspd/ftz038.
Pseudomonas aeruginosa is an important opportunistic pathogen normally associated with increasing morbidity and mortality of immunocompromised hosts with respiratory infections. The phenotypic and genetic features of P. aeruginosa from patients with chronic obstructive pulmonary disease (COPD) remain poorly understood. By using the sputum samples of 25 hospitalized COPD patients from the affiliated hospital of Southwest Medical University (China), we identified a P. aeruginosa isolate, COP2, which showed multiple antibiotic resistance and enhanced Pseudomonas quinolone signal (PQS) production but decreased motility, biofilm formation and virulence compared with the model strain PAO1. Importantly, COP2 harbored a substantial amount of mutations that might influence the functions of 1771 genes in the genome and the evolutionary status of this isolate was clearly distinct from the PAO1 lineage. Accordingly, COP2 had a discrepant transcriptional pattern relating to flagellar assembly, antibiotic resistance, biofilm and PQS production, and can increase the capacities of compound degradation in response to resource/space stresses. Therefore, the identification of COP2 in this study provides preliminary information regarding the genetic features and survival strategy of P. aeruginosa in colonizing COPD lungs and lays the foundations for further understanding of the pathogenic mechanisms of pseudomonal infections.
铜绿假单胞菌是一种重要的机会致病菌，通常与呼吸道感染的免疫功能低下宿主的发病率和死亡率增加有关。慢性阻塞性肺疾病（COPD）患者的铜绿假单胞菌的表型和遗传特征仍知之甚少。本研究通过使用来自中国西南医科大学附属医院的 25 例住院 COPD 患者的痰样本，鉴定出一株铜绿假单胞菌分离株 COP2，与模式株 PAO1 相比，该分离株表现出多种抗生素耐药性和增强的假单胞菌喹诺酮信号（PQS）产生，但运动性、生物膜形成和毒力降低。重要的是，COP2 携带大量可能影响基因组中 1771 个基因功能的突变，并且该分离株的进化地位与 PAO1 谱系明显不同。因此，COP2 的转录模式与鞭毛组装、抗生素耐药性、生物膜和 PQS 产生有关，并且可以响应资源/空间胁迫增加化合物降解的能力。因此，本研究中 COP2 的鉴定为定植于 COPD 肺部的铜绿假单胞菌的遗传特征和生存策略提供了初步信息，并为进一步了解假单胞菌感染的发病机制奠定了基础。
