激光照射对体外培养婴儿血管瘤内皮细胞生长因子和细胞凋亡的影响。
Effects of laser irradiation on growth factors and cell apoptosis of in vitro cultured infant hemangioma endothelial cells.
机构信息
Department of Dermatology, The first affiliated hospital of Xinjiang Medical University, Urumqi 830054, China.
Department of Dermatology, The first affiliated hospital of Xinjiang Medical University, Urumqi 830054, China.
出版信息
Life Sci. 2019 Sep 15;233:116685. doi: 10.1016/j.lfs.2019.116685. Epub 2019 Jul 23.
AIMS
The present study aimed to investigate the effects of laser irradiation on the growth factors and cell apoptosis of in vitro cultured infant hemangioma endothelial cells.
MAIN METHODS
Endothelial cells of infant hemangioma were cultured in vitro and irradiated using a variable pulse width 1064 nm Nd:YAG laser and intense pulsed light (IPL), the expression of VEGF, VEGFR-2, bFGF and their mRNAs before and after irradiation were measured by ELISA, western blot, RT-PCR and flow cytometry, and changes in the apoptotic rate of endothelial cells in hemangioma were monitored.
KEY FINDINGS
The mRNA and protein expressions of VEGF, VEGFR-2, bFGF in hemangioma endothelial cells were inhibited by both Nd:YAG laser and ILP compared to the control cells. The apoptotic rates of hemangioma endothelial cells were also decreased after both laser irradiation treatments in comparison to the blank group. The differences were statistically significant.
SIGNIFICANCE
Laser irradiation treats hemangioma not only through a selective photothermal mechanism, but also through cytokine signaling pathways.
目的
本研究旨在探讨激光辐照对体外培养婴儿血管瘤内皮细胞生长因子和细胞凋亡的影响。
主要方法
体外培养婴儿血管瘤内皮细胞，采用可变脉宽 1064nmNd：YAG 激光和强脉冲光（IPL）辐照，辐照前后采用 ELISA、western blot、RT-PCR 和流式细胞术检测 VEGF、VEGFR-2、bFGF 的表达及其 mRNA，监测血管瘤内皮细胞凋亡率的变化。
主要发现
与对照组相比，Nd：YAG 激光和 IPL 均可抑制血管瘤内皮细胞中 VEGF、VEGFR-2、bFGF 的 mRNA 和蛋白表达。与空白组相比，激光照射后血管瘤内皮细胞的凋亡率也降低，差异有统计学意义。
意义
激光照射治疗血管瘤不仅通过选择性光热机制，还通过细胞因子信号通路。
Zotero 插件