Genecology Research Centre, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, Queensland 4556, Australia; Research Institute for Marine Fisheries (RIMF), 224 LeLai Street, HaiPhong City, Viet Nam.
Genecology Research Centre, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, Queensland 4556, Australia.
Gen Comp Endocrinol. 2019 Nov 1;283:113229. doi: 10.1016/j.ygcen.2019.113229. Epub 2019 Jul 23.
Neuropeptides play important roles in the regulation of physiological processes such as growth, metabolism and reproduction. In sea cucumbers (Phylum Echinodermata), numerous neuropeptides have been identified and some are attributed to reproductive processes. In this study, our goal was to gain a better understanding of the neuropeptide repertoire for the black sea cucumber Holothuria leucospilota, a species that has been severely overfished from the wild due to human consumption. We applied in silico transcriptome analysis of the adult H. leucospilota radial nerve cord, gonad and body wall to elucidate 35 neuropeptides that are conserved throughout the Bilateria. Then, liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of radial nerve cord was employed and showed an additional 8 putative novel neuropeptide precursors, whose predicative cleaved peptides do not share sequence similarity with any reported neuropeptides. These data provide an important basis for experimental approaches to manipulate H. leucospilota broodstock reproduction and growth in culture, which will hopefully re-establish population numbers.
神经肽在生长、代谢和繁殖等生理过程的调节中发挥着重要作用。在海参（棘皮动物门）中，已经鉴定出许多神经肽，其中一些与生殖过程有关。在这项研究中，我们的目标是更好地了解黑海参（Holothuria leucospilota）的神经肽谱，由于人类食用，这种海参已在野外被过度捕捞。我们应用计算机转录组分析成年黑海参的放射神经索、性腺和体壁，以阐明在双边动物中保守的 35 种神经肽。然后，对放射神经索进行液相色谱串联质谱（LC-MS/MS）分析，显示了另外 8 种推测的新型神经肽前体，其预测的裂解肽与任何报道的神经肽没有序列相似性。这些数据为实验方法提供了重要依据，可用于操纵黑海参亲体繁殖和在培养中生长，有望重建种群数量。
