UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
J Biotechnol. 2019 Sep 10;303:30-36. doi: 10.1016/j.jbiotec.2019.07.007. Epub 2019 Jul 23.
Komagataella pastoris was recently proposed as a source of valuable polysaccharides, namely, the co-polymer chitin-glucan complex (CGC) and mannose-containing polymers (mannans), that are extracted from its cell-wall. In this study, a novel cultivation medium, Medium K, was developed envisaging the simultaneous production of both types of cell-wall polysaccharides. The use of Medium K for the cultivation of K. pastoris resulted in high contents of CGC (19 wt%) and mannans (21 wt%) in the biomass, corresponding to significantly higher products' volumetric productivities (17.5 and 19.2 g/L day, respectively) compared to previous studies. The produced CGC had a chitin:β-glucan molar ratio of 12:88, similarly to previously reported values for K. pastoris CGC (11:89-19:81), while the mannans were mainly composed of mannose units, with a protein content of 10 wt%. These results demonstrated that the developed optimized medium was suitable for cultivation of K. pastoris and the production of both CGC and mannans. It comprised fewer components with considerably reduced salts content, thus representing a significant simplification of the bioprocess with no precipitation problems, without impacting on the polymers' composition.
毕赤酵母最近被提议作为有价值多糖的来源，即壳聚糖-葡聚糖共聚物 (CGC) 和含有甘露糖的聚合物（甘露聚糖），这些多糖从其细胞壁中提取。在这项研究中，开发了一种新型培养基 Medium K，旨在同时生产这两种细胞壁多糖。使用 Medium K 培养毕赤酵母可使生物质中 CGC（19wt%）和甘露聚糖（21wt%）的含量高，与以前的研究相比，产物的比生产率分别显著提高了 17.5 和 19.2g/L/天。所产生的 CGC 的壳聚糖：β-葡聚糖摩尔比为 12:88，与先前报道的毕赤酵母 CGC 的摩尔比（11:89-19:81）相似，而甘露聚糖主要由甘露糖单元组成，蛋白质含量为 10wt%。这些结果表明，开发的优化培养基适合于毕赤酵母的培养以及 CGC 和甘露聚糖的生产。它包含更少的成分，盐含量大大降低，因此简化了生物过程，没有沉淀问题，对聚合物的组成没有影响。
