详细分析密度梯度离心法分离银纳米粒子的大小。
Detailed analysis of size-separation of silver nanoparticles by density gradient centrifugation method.
机构信息
Semiconductor Department, Photonic Research Center, Graduate University of Advanced Technology, PO Box 76315-117, Kerman, Iran.
New Materials Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, PO Box 76315-117, Kerman, Iran.
出版信息
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109817. doi: 10.1016/j.msec.2019.109817. Epub 2019 May 29.
In this research, silver nanoparticles were synthesized by chemical precipitation method and stabilized by chitosan biopolymer in the range of 15 to 235 nm. Then, the silver nanoparticles were separated by density gradient centrifugation method at different gradients and centrifuged at various duration and speed. The best separation was done with the gradient chosen at 10%, 20%, 30% and 40% for a duration of 2 h at 6000 rpm. The UV-visible spectra demonstrated the proper synthesis of silver nanoparticles, while FTIR spectrum and XRD data revealed the structure of prepared Ag-NPs. The FESEM and TEM analysis were used to check for the exact shape and size of Ag-NPs, respectively. However, the separation of silver nanoparticles was assessed making use of DLS analysis.
在这项研究中，通过化学沉淀法合成了银纳米粒子，并在 15 至 235nm 的范围内用壳聚糖生物聚合物稳定。然后，通过密度梯度离心法在不同梯度下分离银纳米粒子，并在不同的时间和速度下离心。最佳分离是在梯度为 10%、20%、30%和 40%的条件下进行，时间为 2 小时，转速为 6000rpm。紫外-可见光谱表明了银纳米粒子的正确合成，而傅里叶变换红外光谱和 X 射线衍射数据则揭示了所制备的 Ag-NPs 的结构。FESEM 和 TEM 分析分别用于检查 Ag-NPs 的精确形状和尺寸。然而，利用 DLS 分析评估了银纳米粒子的分离情况。
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