Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, China.
The Sixteenth Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, China.
J Plast Reconstr Aesthet Surg. 2019 Oct;72(10):1661-1668. doi: 10.1016/j.bjps.2019.06.006. Epub 2019 Jul 5.
Hydrogen gas exists in the atmosphere and was previously considered an inert gas. It has been reported to have protective effects on tissue ischemia-reperfusion (IR) injuries in animal models. The protective mechanism of hydrogen molecules is based on selectively reducing highly strong oxidants in cells, thereby reducing inflammation and decreasing the contents of MDA, FOXO3a, and other pathways that result in flap necrosis. Previous studies were conducted with postconditioning with hydrogen. In this article, we want to investigate whether inhalation of hydrogen has a preventive effect on IR injury.
Forty-five adult male Sprague Dawley rats (body weight 220-250 g) were randomly divided into three groups: (1) Sham operation group (SH), (2) Ischemia-reperfusion injury group (IR), and (3) Ischemia-reperfusion injury with preconditioning hydrogen group (PRH). IR injury was induced by clamping the right superficial epigastric artery for 3 h. Before undergoing 3 h of IR management, the PRH group was treated with hydrogen inhalation for 1 h. On the third postoperative day, survival area and blood perfusion of the flap were assessed using laser Doppler flowmetry. RIP1 and RIP3 were examined by immunological detection and western blot analysis.
Both the IR and PRH groups had less skin flap survival area and less blood perfusion than the sham group (P < 0.05). RIP1 and RIP3 were highly expressed in the IR and PRH groups when compared with those in the SH group (P < 0.05). There were no significant differences in flap survival rate (32.34 ± 2.19% and 33.09 ± 1.64%), average blood perfusion (41.66 ± 3.53 pu, 48.57 ± 2.83 pu), and expression of RIP1 and RIP3 (0.5167 ± 0.1409 and 0.4693 ± 0.1454) between the IR and PRH groups.
Preconditioning with one-time inhaled hydrogen does not attenuate skin flap IR injuries in rat models.
氢气存在于大气中，以前被认为是一种惰性气体。据报道，它对动物模型中的组织缺血再灌注（IR）损伤具有保护作用。氢分子的保护机制基于选择性地减少细胞内的强氧化剂，从而减少炎症并降低 MDA、FOXO3a 等导致皮瓣坏死的途径的含量。以前的研究是用后处理进行的。在本文中，我们想研究吸入氢气是否对 IR 损伤有预防作用。
将 45 只成年雄性 Sprague Dawley 大鼠（体重 220-250g）随机分为三组：（1）假手术组（SH），（2）缺血再灌注损伤组（IR），和（3）缺血再灌注损伤预处理氢气组（PRH）。通过夹闭右侧腹壁浅动脉 3 小时诱导 IR 损伤。在接受 3 小时 IR 处理之前，PRH 组接受氢气吸入 1 小时。术后第 3 天，使用激光多普勒血流仪评估皮瓣的存活面积和血液灌注。通过免疫检测和 Western blot 分析检测 RIP1 和 RIP3。
与 SH 组相比，IR 组和 PRH 组的皮瓣存活面积和血液灌注均减少（P<0.05）。与 SH 组相比，IR 组和 PRH 组的 RIP1 和 RIP3 表达均升高（P<0.05）。两组间皮瓣存活率（32.34±2.19%和 33.09±1.64%）、平均血液灌注量（41.66±3.53pu，48.57±2.83pu）和 RIP1 和 RIP3 的表达（0.5167±0.1409 和 0.4693±0.1454）均无统计学差异。
单次吸入氢气预处理不能减轻大鼠模型皮肤瓣 IR 损伤。
