Department of Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Düsseldorf, Germany.
Department of Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Düsseldorf, Germany.
World Neurosurg. 2019 Nov;131:e271-e276. doi: 10.1016/j.wneu.2019.07.136. Epub 2019 Jul 24.
With the introduction of the 5-aminolevulinic acid (5-ALA) technique, surgical neuro-oncology has made a major advance. 5-ALA fluorescence-guided resection of malignant glioma results in more complete surgical resections and subsequently prolonged survival. However, it remains uncertain how light intensities of the blue light source and 5-ALA-derived fluorescence intensities of the illuminated tissue are connected. The aim of the present study was to compare light intensities of different blue light sources and protoporphyrin (PpIX) fluorescence intensities of PpIX solutions with defined concentrations after illumination with different light sources.
The light spectrum of 7 different blue light sources and the fluorescence intensity of 2 PpIX solutions (0.15 μg/mL and 5 μg/mL) were quantified after illumination. We compared the Zeiss OPMI Pentero microscope, the Zeiss OPMI Pentero 900 microscope, the Leica M530 OH6 microscope, an endoscope equipped with the 5-ALA technique, a mini-spectrometer equipped with a multi-channel light-emitting diode (LED) source emitting monochromatic light, a modified commercially available LED head lamp, and a commercially available unmodified UV-LED lamp. PpIX fluorescence was quantified in a standardized setup using a mini-spectrometer.
Maximum light intensities of the evaluated light sources were reached at different wavelengths. All tested devices were able to detect PpIX-induced fluorescence. However, the intensity of PpIX fluorescence of the differently concentrated PpIX solutions (0.15 μg/mL and 5 μg/mL) was significantly dependent on the light source used.
Intensity of the 5-ALA-derived fluorescence is related to the light source used.
随着 5-氨基酮戊酸（5-ALA）技术的引入，外科神经肿瘤学取得了重大进展。5-ALA 荧光引导切除恶性胶质瘤可实现更彻底的手术切除，从而延长生存时间。然而，目前尚不清楚蓝光光源的光强度与被照射组织的 5-ALA 衍生荧光强度之间的关系。本研究旨在比较不同蓝光光源的光强度和不同光源照射后具有定义浓度的原卟啉（PpIX）溶液的 PpIX 荧光强度。
定量测定了 7 种不同蓝光光源的光谱和 2 种 PpIX 溶液（0.15μg/mL 和 5μg/mL）的荧光强度。我们比较了蔡司 OPMI Pentero 显微镜、蔡司 OPMI Pentero 900 显微镜、徕卡 M530 OH6 显微镜、配备 5-ALA 技术的内窥镜、配备多通道发光二极管（LED）光源发射单色光的微型光谱仪、经过改装的市售 LED 头灯和市售未经改装的 UV-LED 灯。使用微型光谱仪在标准化设置中定量测定 PpIX 荧光。
评估的光源的最大光强度出现在不同的波长。所有测试的设备都能够检测到 PpIX 诱导的荧光。然而，不同浓度的 PpIX 溶液（0.15μg/mL 和 5μg/mL）的 PpIX 荧光强度显著依赖于所使用的光源。
5-ALA 衍生荧光的强度与所使用的光源有关。
Zotero 插件