Carsons S E, Schwartzman S, Diamond H S, Berkowitz E
Department of Medicine, SUNY Stony Brook.
Clin Exp Immunol. 1988 Apr;72(1):37-42.
The interaction between fibronectin and C1q was studied in the presence of normal human plasma and rheumatoid synovial fluid by solid phase binding assay. Fibronectin-C1q binding occurred in the presence of rheumatoid synovial fluid but not in the presence of normal plasma. Binding was strongest at 4 degrees C and in the presence of EDTA. Fibronectin-C1q binding could be induced in the presence of normal plasma by hypotonicity, augmentation of the concentration of solution-phase fibronectin or by the addition of heat-aggregated IgG. The C1q present in rheumatoid synovial fluid bound to both aminoterminal collagen-binding and carboxyterminal noncollagen binding fibronectin fragments although binding to the aminoterminal fragment was stronger. The interaction between fibronectin and C1q in rheumatoid synovial fluid may modulate immune-complex deposition and complement activation in the inflamed joint.
通过固相结合试验，在正常人血浆和类风湿性滑液存在的情况下研究了纤连蛋白与C1q之间的相互作用。纤连蛋白与C1q的结合发生在类风湿性滑液存在时，而在正常血浆存在时不发生。在4℃且存在乙二胺四乙酸（EDTA）的情况下结合最强。通过低渗、增加溶液相纤连蛋白浓度或添加热聚集的免疫球蛋白G（IgG），可在正常血浆存在时诱导纤连蛋白与C1q的结合。类风湿性滑液中存在的C1q与氨基末端胶原结合和羧基末端非胶原结合的纤连蛋白片段均结合，尽管与氨基末端片段的结合更强。类风湿性滑液中纤连蛋白与C1q之间的相互作用可能调节炎症关节中的免疫复合物沉积和补体激活。
