咖啡因不会使具有功能性钙储存的去皮子宫纤维收缩。

Caffeine does not contract skinned uterine fibers with a functional Ca store.

作者信息

Savineau J P

机构信息

Laboratoire de Physiologie Cellulaire, Université de Bordeaux, France.

出版信息

Eur J Pharmacol. 1988 Apr 27;149(1-2):187-90. doi: 10.1016/0014-2999(88)90062-3.

DOI:10.1016/0014-2999(88)90062-3

PMID:3135196

Abstract

Chemically skinned fibers were prepared from pregnant rat myometrium. In the presence of a low EGTA concentration (0.1 mM), caffeine (0.1-50 mM) produced no contraction whereas both inositol-1,4,5,-triphosphate and the ionophore A23187 evoked contractile responses insensitive to cyanide and azide. Furthermore, caffeine antagonized the maximal contractions induced by pCa = 5 (in the presence of 4 mM EGTA). It is concluded that caffeine inhibits some mechanism of the contraction in rat myometrium and thus that caffeine is not a good tool for the study of intracellular Ca stores in this smooth muscle.

摘要

从怀孕大鼠的子宫肌层制备化学去表皮纤维。在低EGTA浓度（0.1 mM）存在的情况下，咖啡因（0.1 - 50 mM）不会引起收缩，而肌醇-1,4,5-三磷酸和离子载体A23187均能引起对氰化物和叠氮化物不敏感的收缩反应。此外，咖啡因拮抗了pCa = 5（在4 mM EGTA存在的情况下）诱导的最大收缩。得出的结论是，咖啡因抑制大鼠子宫肌层收缩的某些机制，因此咖啡因不是研究这种平滑肌细胞内钙储存的良好工具。