Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China, Agricultural University, Guangzhou, 510642, China.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Fish Shellfish Immunol. 2019 Oct;93:308-312. doi: 10.1016/j.fsi.2019.07.065. Epub 2019 Jul 25.
Initiation of the innate immune response requires recognition of pathogen-associated molecular patterns by pathogen recognition receptors such as Toll-like receptors (TLRs). MyD88 adaptor-like (Mal) is an adaptor that responds to TLR activation and acts as a bridging adaptor for MyD88. In the present study, the open reading frame of Mal was identified in orange-spotted grouper (Epinephelus coioides), and named EcMal. It contained 831 bp encoding 276 aa, and was encoded by a 1299 bp DNA sequence with three exons and two introns. EcMal and the Mal sequence of other species shared different degrees of sequence identity, and clustered into the same group. EcMal was distributed in all tissues tested in healthy grouper, with the highest expression level in the head kidney. After infection with Cryptocaryon irritans, the expression level of EcMal was up-regulated in the gill and spleen. In addition, EcMal exhibited global cytosolic and nucleus localization, and could significantly activate NF-κB activity in grouper spleen cells.
先天免疫反应的启动需要病原体识别受体（如 Toll 样受体（TLRs））识别病原体相关分子模式。MyD88 衔接子样（Mal）是一种对 TLR 激活作出反应的衔接子，充当 MyD88 的桥接衔接子。在本研究中，在橙色斑点石斑鱼（Epinephelus coioides）中鉴定出 Mal 的开放阅读框，并将其命名为 EcMal。它包含编码 276 个氨基酸的 831bp，由编码 1299bp DNA 序列的三个外显子和两个内含子组成。EcMal 和其他物种的 Mal 序列具有不同程度的序列同一性，并聚类到同一组。EcMal 分布在健康石斑鱼检测的所有组织中，在头肾中的表达水平最高。感染 Cryptocaryon irritans 后，EcMal 在鳃和脾脏中的表达水平上调。此外，EcMal 表现出全细胞质和细胞核定位，并能显著激活石斑鱼脾脏细胞中的 NF-κB 活性。
