Willson Jessica A, Damjanovski Sashko
Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada.
Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada.
Gene Expr Patterns. 2019 Dec;34:119066. doi: 10.1016/j.gep.2019.119066. Epub 2019 Jul 26.
Proper cell-cell and cell-ECM interactions are vital for cell migration and patterning of the vertebrate embryo. MMPs and their inhibitors, RECK and TIMPs, are all differentially expressed during embryogenesis to regulate such ECM remodeling and cell interactions. MT1-MMP, RECK, and TIMP-2 are unique amongst other ECM-regulating proteins as they act in the pericellular space. Past studies have shown that RECK and TIMP-2 interact with MT1-MMP on the cell surface, thereby influencing cell behaviour as well as the microenvironment immediately surrounding the cells. We investigated the localization of RECK, TIMP-2, and MT1-MMP proteins throughout early X. laevis development using immunohistochemistry. We found that during neural tube formation, axis elongation, and organogenesis, RECK, TIMP-2, and MT1-MMP proteins show highly similar localization patterns, particularly in the ectoderm and in the dorsal-ventral differentiation of the neural tube. Our data suggests they function together during patterning events in early Xenopus development.
适当的细胞间和细胞与细胞外基质（ECM）的相互作用对于脊椎动物胚胎的细胞迁移和模式形成至关重要。基质金属蛋白酶（MMPs）及其抑制剂RECK和组织金属蛋白酶抑制剂（TIMPs）在胚胎发生过程中均有差异表达，以调节这种细胞外基质重塑和细胞相互作用。MT1-MMP、RECK和TIMP-2在其他细胞外基质调节蛋白中是独特的，因为它们在细胞周围空间起作用。过去的研究表明，RECK和TIMP-2在细胞表面与MT1-MMP相互作用，从而影响细胞行为以及细胞周围的微环境。我们使用免疫组织化学研究了RECK、TIMP-2和MT1-MMP蛋白在非洲爪蟾早期发育过程中的定位。我们发现，在神经管形成、轴伸长和器官发生过程中，RECK、TIMP-2和MT1-MMP蛋白表现出高度相似的定位模式，特别是在外胚层以及神经管的背腹分化中。我们的数据表明它们在非洲爪蟾早期发育的模式形成事件中共同发挥作用。
