Judd A M, Ross P C, Spangelo B L, MacLeod R M
Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Mol Cell Endocrinol. 1988 May;57(1-2):115-21. doi: 10.1016/0303-7207(88)90040-8.
The effects of angiotensin II (AII) on prolactin release and arachidonate liberation were studied in anterior pituitary cells preincubated with [3H]arachidonate to label the cellular phospholipids. AII increased prolactin release and [3H]arachidonate liberation over similar concentration ranges with the dynamics of these two events proving identical. Dopamine attenuated both prolactin release and [3H]arachidonate liberation. The diacylglycerol lipase inhibitor RHC80267 decreased AII-stimulated prolactin release and arachidonate liberation. Further evidence that AII-induced release of arachidonate is mediated by a diacylglycerol lipase is suggested by the finding that AII increased [14C]stearate liberation from cells prelabeled with the fatty acid. Although arachidonate itself may have some role in prolactin secretion, it is likely that arachidonate metabolites are more directly involved because BW755c and AA861, inhibitors of arachidonate metabolite formation, increased AII-stimulated arachidonate liberation, but decreased prolactin release.
在预先用[3H]花生四烯酸孵育以标记细胞磷脂的垂体前叶细胞中，研究了血管紧张素II（AII）对催乳素释放和花生四烯酸释放的影响。在相似的浓度范围内，AII增加了催乳素释放和[3H]花生四烯酸释放，这两个事件的动力学证明是相同的。多巴胺减弱了催乳素释放和[3H]花生四烯酸释放。二酰基甘油脂肪酶抑制剂RHC80267降低了AII刺激的催乳素释放和花生四烯酸释放。AII诱导的花生四烯酸释放由二酰基甘油脂肪酶介导的进一步证据来自以下发现：AII增加了预先用脂肪酸标记的细胞中[14C]硬脂酸的释放。尽管花生四烯酸本身可能在催乳素分泌中起一定作用，但花生四烯酸代谢产物可能更直接地参与其中，因为花生四烯酸代谢产物形成的抑制剂BW755c和AA861增加了AII刺激的花生四烯酸释放，但降低了催乳素释放。
