Verhoeven G, Cailleau J
Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Department of Developmental Biology, Leuven, Belgium.
Mol Cell Endocrinol. 1988 May;57(1-2):61-7. doi: 10.1016/0303-7207(88)90032-9.
We studied the effects of androgens on basal and FSH-stimulated aromatase activity in Sertoli cell-enriched monolayers. Daily exposure to androgens from the first day of culture results in a time- and dose-dependent decrease in inducible aromatase activity. The inhibition is observed whether FSH, L-isoproterenol or (Bu)2cAMP is used as inducer of the aromatase activity. Basal activity is not affected by preincubation with androgens and the inhibitory effect is not observed after short-term exposure (24 h) to these hormones. The ability of different androgens to decrease inducible aromatase activity does not depend on their ability to serve as a substrate for the aromatase but parallels their ability to stimulate the production of androgen-binding protein. Moreover, the effect of testosterone is neutralized by the antiandrogen cyproterone acetate, suggesting that it is mediated by the androgen receptor. These data suggest that testicular androgens may be responsible for the decrease in FSH-inducible aromatase activity observed in intact rats between days 10 and 30 of life. Similarly, the removal of these androgens during the preparation of Sertoli cell cultures may explain the spontaneous increase in inducible aromatase activity observed when these cultures are maintained in the absence of androgens.
我们研究了雄激素对富含支持细胞的单层培养物中基础和促卵泡激素（FSH）刺激的芳香化酶活性的影响。从培养第一天开始每日暴露于雄激素会导致诱导型芳香化酶活性出现时间和剂量依赖性降低。无论使用FSH、L-异丙肾上腺素还是（Bu）2cAMP作为芳香化酶活性的诱导剂，均观察到这种抑制作用。基础活性不受与雄激素预孵育的影响，且短期暴露（24小时）于这些激素后未观察到抑制作用。不同雄激素降低诱导型芳香化酶活性的能力并不取决于它们作为芳香化酶底物的能力，而是与其刺激雄激素结合蛋白产生的能力平行。此外，睾酮的作用可被抗雄激素醋酸环丙孕酮中和，表明其作用是由雄激素受体介导的。这些数据表明，睾丸雄激素可能是导致出生后10至30天完整大鼠中FSH诱导的芳香化酶活性降低的原因。同样，在支持细胞培养物制备过程中去除这些雄激素可能解释了在无雄激素条件下维持这些培养物时观察到的诱导型芳香化酶活性的自发增加。
