Müller C, Knoflach P, Smetana P, Meisinger V, Zielinski C C
Second Department of Gastroenterology and Hepatology, University Hospital, Vienna, Austria.
Digestion. 1988;39(1):26-34. doi: 10.1159/000199604.
Natural killer cell (NK) activity and antibody-dependent cellular cytotoxicity (ADCC) exerted by peripheral blood mononuclear cells (PMNC) were investigated in 53 patients with Crohn's disease (CD). NK activity and ADCC were found to be significantly reduced in patients with CD (p less than 0.0005) as compared to healthy controls. Both effector cell functions increased after in vitro treatment of PMNC with gamma-interferon, but did not reach the levels found in controls (p less than 0.0005). Neither NK activity nor ADCC was significantly influenced by therapy with corticosteroids. Moreover, the reduced serum zinc levels in patients with CD, which have been shown to be associated with impaired immune function, did not influence the lytic effector cell mechanism assayed either. Finally, no association could be found between NK cell activity or ADCC and CD activity index, the extent of the disease and several laboratory parameters of inflammation. We conclude that patients with CD have a reduced lytic effector cell function which remains uninfluenced by corticosteroid treatment and seems to be present independently of disease activity.
对53例克罗恩病（CD）患者外周血单个核细胞（PMNC）的自然杀伤细胞（NK）活性及抗体依赖性细胞毒性（ADCC）进行了研究。结果发现，与健康对照相比，CD患者的NK活性和ADCC显著降低（p<0.0005）。用γ干扰素体外处理PMNC后，两种效应细胞功能均增强，但未达到对照水平（p<0.0005）。皮质类固醇治疗对NK活性和ADCC均无显著影响。此外，CD患者血清锌水平降低与免疫功能受损有关，但也未影响所检测的溶细胞效应细胞机制。最后，未发现NK细胞活性或ADCC与CD活动指数、疾病范围及多项炎症实验室指标之间存在关联。我们得出结论，CD患者的溶细胞效应细胞功能降低，不受皮质类固醇治疗影响，且似乎独立于疾病活动存在。
