Robbins P A, Maino V C, Warner N L, Brodsky F M
Becton Dickinson Immunocytometry Systems, Mountain View, CA 94043.
J Immunol. 1988 Aug 15;141(4):1281-7.
The expression of human histocompatibility class II Ag was measured on activated T cells and monocytes by quantitative mAb binding in direct two-color immunofluorescence. Monocytes activated by IFN-gamma bound an average of 2 x 10(6) DR-specific mAb, 3 x 10(5) DQ-specific mAb, and 7 x 10(5) DP-specific mAb per cell. For T cells activated by anti-CD3, a subpopulation bound 1 x 10(5) DR-specific mAb, 5 x 10(4) DQ-specific mAb and 5 x 10(4) DP-specific mAb per cell. These measurements were obtained after establishing a base line of class II Ag expression on resting B cells and monocytes. Resting B cells and those monocytes that were positive for class II Ag bound identical amounts of mAb; 3 x 10(4) DR-specific mAb, 3 x 10(3) DQ-specific mAb and 2 x 10(4) DP-specific mAb. However, most resting monocytes (75%) expressed only DR Ag. In the process of studying the expression of class II Ag on T cells, it was necessary to define and analyze the activated T cell state. Cell cultures activated with 0.3 ng/ml anti-CD3 had the highest expression of class II Ag on T cells, whereas those activated with 3.0 ng/ml anti-CD3 had the highest expression of IL-2R on T cells. Addition of IL-2 had no further effect on DR Ag expression on T cells but did up-regulate IL-2R expression. Reducing the initial monocyte concentration before activating T cells increased class II Ag expression on T cells without affecting IL-2R expression. The results obtained on T cell activation suggest that perhaps a lymphokine may be made by CD3-activated T cells which induces class II Ag expression on T cells.
通过直接双色免疫荧光中的定量单克隆抗体结合，测定了活化T细胞和单核细胞上人类组织相容性II类抗原的表达。经γ干扰素活化的单核细胞，平均每个细胞结合2×10⁶个DR特异性单克隆抗体、3×10⁵个DQ特异性单克隆抗体和7×10⁵个DP特异性单克隆抗体。对于经抗CD3活化的T细胞，一个亚群每个细胞结合1×10⁵个DR特异性单克隆抗体、5×10⁴个DQ特异性单克隆抗体和5×10⁴个DP特异性单克隆抗体。这些测量结果是在确定了静息B细胞和单核细胞上II类抗原表达的基线后获得的。静息B细胞和那些II类抗原呈阳性的单核细胞结合相同数量的单克隆抗体；3×10⁴个DR特异性单克隆抗体、3×10³个DQ特异性单克隆抗体和2×10⁴个DP特异性单克隆抗体。然而，大多数静息单核细胞（75%）仅表达DR抗原。在研究T细胞上II类抗原表达的过程中，有必要定义和分析活化T细胞状态。用0.3 ng/ml抗CD3活化的细胞培养物中，T细胞上II类抗原的表达最高，而用3.0 ng/ml抗CD3活化的细胞培养物中，T细胞上IL-2R的表达最高。添加IL-2对T细胞上DR抗原的表达没有进一步影响，但确实上调了IL-2R的表达。在活化T细胞之前降低初始单核细胞浓度，可增加T细胞上II类抗原的表达，而不影响IL-2R的表达。关于T细胞活化的结果表明，也许CD3活化的T细胞可产生一种淋巴因子，诱导T细胞上II类抗原的表达。
