Fang Li, Ren Dayong, Wang Zuhao, Liu Chunlei, Wang Ji, Min Weihong
College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.
National Engineering Laboratory on Wheat and Corn Further Processing, Changchun, China.
J Food Biochem. 2019 Mar;43(3):e12722. doi: 10.1111/jfbc.12722. Epub 2018 Dec 9.
The crude protein hydrolysates of wild hazel have good immunoregulation and antioxidation effects. However, the components responsible for their antioxidation effect remain unknown. In this study, six antioxidative peptides (EW, DWDPK, ADGF, SGAF, ETTL, and AGGF) were tested for their protective effects on oxidative stress injury in human umbilical vein endothelial cells (HUVECs). The results demonstrated that the six peptides are nontoxic and have a protective effect on oxidative stress injury induced by Ang II. Three peptides (EW, ADGF, and DWDPK) inhibited the morphological changes, downregulated the content of lactate dehydrogenase and malondialdehyde, upregulated the activity of antioxidant enzymes catalase, total superoxide dismutase and glutathione peroxidase, and scavenged reactive oxygen species (ROS) in HUVECs. Quantitative reverse transcriptive polymerase chain reaction and western blot assays indicated that these three peptides regulated NADPH oxidase activity and ROS production by reducing NOX4 and p22phox levels. Overall, they have a significant protective effect against oxidative stress injury and have potential application in developing new functional foods. PRACTICAL APPLICATIONS: Corylus heterophylla Fisch is a good quality wild hazel distributed in Northeast China. Wild hazelnut of the species C. heterophylla Fisch was selected as experimental object and has high nutritive values and have abundant proteins (20%-30%), fats (40%-50%), carbohydrates (13%-24%), dietary fibers (8.2%-9.6%), vitamins, and micronutrients. Our results indicate that hazelnut peptides (EW, ADGF, and DWDPK) can ensure normal growth of cells by protecting important antioxidant enzyme systems, by enhancing antioxidant defense, by directly affecting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and by reducing intracellular reactive oxygen species (ROS) production in HUVECs, indicating that the three antioxidative peptides have a protective effect against Ang II-induced oxidative stress injury. Therefore, the antioxidative peptides from C. heterophylla Fisch may be a promising candidate for functional food ingredients and/or pharmaceuticals.
野生榛子的粗蛋白水解物具有良好的免疫调节和抗氧化作用。然而，其抗氧化作用的成分尚不清楚。在本研究中，测试了六种抗氧化肽（EW、DWDPK、ADGF、SGAF、ETTL和AGGF）对人脐静脉内皮细胞（HUVECs）氧化应激损伤的保护作用。结果表明，这六种肽无毒，对血管紧张素II诱导的氧化应激损伤具有保护作用。三种肽（EW、ADGF和DWDPK）抑制了形态变化，下调了乳酸脱氢酶和丙二醛的含量，上调了抗氧化酶过氧化氢酶、总超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，并清除了HUVECs中的活性氧（ROS）。定量逆转录聚合酶链反应和蛋白质印迹分析表明，这三种肽通过降低NOX4和p22phox水平来调节NADPH氧化酶活性和ROS产生。总体而言，它们对氧化应激损伤具有显著的保护作用，在开发新型功能性食品方面具有潜在应用价值。实际应用：平榛是分布于中国东北地区的优质野生榛子。选取平榛野生榛子作为实验对象，其营养价值高，含有丰富的蛋白质（20%-30%）、脂肪（40%-50%）、碳水化合物（13%-24%）、膳食纤维（8.2%-9.6%）、维生素和微量营养素。我们的结果表明，榛子肽（EW、ADGF和DWDPK）可以通过保护重要的抗氧化酶系统、增强抗氧化防御、直接影响烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶活性以及减少HUVECs中细胞内活性氧（ROS）的产生来确保细胞的正常生长，表明这三种抗氧化肽对血管紧张素II诱导的氧化应激损伤具有保护作用。因此，平榛的抗氧化肽可能是功能性食品成分和/或药物的有前途的候选者。
