College of Food Science and Engineering, Ocean University of China, Qingdao, China.
J Food Biochem. 2019 Feb;43(2):e12730. doi: 10.1111/jfbc.12730. Epub 2018 Nov 14.
Methodology to enhance the intestinal absorption of peptides is an important challenge due to their easily degradation and poor permeability across the intestinal epithelium. In this study, the fish-derived peptide (DGDDGEAGKIG)-loaded chitosan (CS) nanoparticles (CS/PEP-NPs) were prepared and investigated in Caco-2 monolayer model. The results indicated zeta potential of CS/PEP-NPs increased with the increase in molecular weight of CS (10-50 kDa). Transmission electron microscopy images revealed the CS/PEP-NPs were uniform spherical-shaped nanoparticles with a diameter of 50-200 nm (150 kDa). Compared to other CS/PEP-NPs, 150-kDa CS/PEP-NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10 cm s ) and cumulative amount of peptide (120 min, 2,987 ng) in Caco-2 cells. CS/PEP-NPs could reduce the tight junction integrity of Caco-2 cells and enhance the intracellular fluorescence intensities of fluorescein isothiocyanate-labeled peptide. These findings suggest that chitosan nanoparticles are promising carriers to promote intestinal absorption of fish-derived peptide via paracellular pathway mediated by tight junctions. PRACTICAL APPLICATIONS: Chitosans are promising carriers to promote intestinal absorption of fish-derived peptide. The 150-kDa CS/PEP-NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10 cm s ) and cumulative amount of peptide (120 min, 2,987 ng) in Caco-2 cells. CS/PEP-NPs could reduce the tight junction integrity of Caco-2 cells and enhance the peptide uptake by paracellular pathway. Chitosan nanoparticles can be developed as vehicles for enhancing the cellular uptake of peptide in food industry.
方法学，以提高肠道吸收的肽是一个重要的挑战，由于其容易降解和较差的通透性穿过肠上皮细胞。在这项研究中，鱼衍生肽（DGDDGEAGKIG）负载壳聚糖（CS）纳米粒子（CS/PEP-NPs）的制备和研究在 Caco-2 单层模型。结果表明，Zeta 电位的 CS/PEP-NPs 增加了与增加分子量的 CS（10-50 kDa）。透射电子显微镜图像显示 CS/PEP-NPs 是均匀的球形纳米粒子，直径为 50-200nm（150 kDa）。与其他 CS/PEP-NPs 相比，150 kDa 的 CS/PEP-NPs 表现出优异的表观渗透系数（Papp，2.29×10-6cm/s）和肽累积量（120min，2987ng）在 Caco-2 细胞中。CS/PEP-NPs 可降低 Caco-2 细胞紧密连接的完整性，并增强异硫氰酸荧光素标记肽的细胞内荧光强度。这些发现表明壳聚糖纳米粒子是有前途的载体，通过紧密连接介导的细胞旁途径促进鱼源肽的肠道吸收。实际应用：壳聚糖是一种很有前途的载体，可以促进鱼源肽的肠道吸收。150 kDa 的 CS/PEP-NPs 表现出优异的表观渗透系数（Papp，2.29×10-6cm/s）和肽累积量（120min，2987ng）在 Caco-2 细胞中。CS/PEP-NPs 可降低 Caco-2 细胞紧密连接的完整性，并增强肽通过细胞旁途径的摄取。壳聚糖纳米粒子可作为增强食品工业中肽细胞摄取的载体。
