Nanda Satyabrata, Hussain Sajid
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 311440, China.
Bioinformation. 2019 Mar 15;15(3):165-171. doi: 10.6026/97320630015165. eCollection 2019.
SQUAMOSA promoter-binding protein-like (SPL) transcription factors play vital roles in various plant physiological processes. Although, the identification of the SPL gene family has been done in C4 grass plants, including rice and maize, the same has not been characterized in the C3 grass species Dichanthelium oligosanthes. In this study, 14 SPL genes were identified in the genome of D. oligosanthes. Gene structure analysis of the identified DoSPLs revealed the similarity and redundancy in their exon/intron organizations. Sequence comparisons within the DoSPLs and along with rice SPLs revealed the putative paralogs and orthologs in D. oligosanthes SPL genes. Phylogenetic analysis clustered the DoSPLs into eight groups along with other plant SPLs. Identification of the conserved SBP motifs in all 14 DoSPLs suggested them to be putative SPLs. In addition, the prediction of sub-cellular localization and associated functions for DoSPLs further supported to be SPL genes. The outcome of this study can serve as a framework for the isolation and functional validation of SPL genes in D. oligosanthes.
类SQUAMOSA启动子结合蛋白（SPL）转录因子在植物的各种生理过程中发挥着至关重要的作用。尽管已经在包括水稻和玉米在内的C4禾本科植物中完成了SPL基因家族的鉴定，但在C3禾本科植物少花双花草中尚未对其进行表征。在本研究中，在少花双花草的基因组中鉴定出了14个SPL基因。对已鉴定的DoSPL进行基因结构分析，揭示了它们外显子/内含子组织中的相似性和冗余性。DoSPL内部以及与水稻SPL的序列比较揭示了少花双花草SPL基因中的推定旁系同源物和直系同源物。系统发育分析将DoSPL与其他植物SPL聚类为八组。在所有14个DoSPL中鉴定出保守的SBP基序，表明它们是推定的SPL。此外，对DoSPL的亚细胞定位和相关功能的预测进一步支持它们是SPL基因。本研究的结果可为少花双花草中SPL基因的分离和功能验证提供框架。
