Leung K, Kim K E, Maurer R A, Landefeld T D
Department of Pharmacology and Developmental and Reproductive Biology, University of Michigan, Ann Arbor 48109.
Mol Endocrinol. 1988 Mar;2(3):272-6. doi: 10.1210/mend-2-3-272.
Cyclic changes in the production of the pituitary gonadotrophic hormones, LH and FSH are essential events in the maintenance of the reproductive system of female mammals. While studies have examined changes in the secretion of LH and FSH during the estrous cycle and demonstrated the importance of these hormones in regulation of ovarian development and gametogenesis, considerably less is known concerning the regulation of the biosynthesis of these hormones. Although initial studies have examined changes in LH subunit mRNA concentrations during the rat and ovine estrous cycles, no information concerning the physiological regulation of FSH beta mRNA concentrations has been available. In the present study we have examined the relationship between pituitary concentrations of LH and FSH subunit mRNAs and the serum concentrations of these gonadotropins. The results demonstrate a very different pattern of change for FSH beta subunit mRNA than that observed for alpha and LH beta subunit mRNAs. In fact, FSH beta mRNA concentration decline substantially during the preovulatory period, reaching minimal values at a time when alpha and LH beta mRNA levels are near maximal. Furthermore, this decline in FSH beta mRNA amounts occurs when serum FSH concentrations are maximal. Thus, FSH beta mRNA concentrations follow a very different pattern than that of serum FSH. In contrast, LH beta mRNA and serum LH concentrations tend to increase at the same time. These findings provide evidence that concentrations of LH beta and FSH beta mRNAs are likely regulated by different mechanisms.
垂体促性腺激素LH和FSH分泌的周期性变化是维持雌性哺乳动物生殖系统的重要事件。虽然已有研究检测了发情周期中LH和FSH分泌的变化，并证明了这些激素在调节卵巢发育和配子发生中的重要性，但对于这些激素生物合成的调节却知之甚少。尽管最初的研究检测了大鼠和绵羊发情周期中LH亚基mRNA浓度的变化，但尚无关于FSHβ mRNA浓度生理调节的信息。在本研究中，我们检测了垂体中LH和FSH亚基mRNA浓度与这些促性腺激素血清浓度之间的关系。结果表明，FSHβ亚基mRNA的变化模式与α和LHβ亚基mRNA的变化模式截然不同。事实上，在排卵前期FSHβ mRNA浓度大幅下降，在α和LHβ mRNA水平接近最大值时达到最小值。此外，FSHβ mRNA量的这种下降发生在血清FSH浓度最高时。因此，FSHβ mRNA浓度的变化模式与血清FSH的变化模式截然不同。相比之下，LHβ mRNA和血清LH浓度往往同时增加。这些发现提供了证据，表明LHβ和FSHβ mRNA的浓度可能受不同机制的调节。
