Liu Xiangye, Li Huiqin, Deng Hongkuan, Zheng Chen, Yan Hongru, Chen Zetian, Bian Anning, Chen Jiaxu, Zheng Kuiyang
Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, China.
School of Life Sciences, Shandong University of Technology, Zibo, China.
Front Vet Sci. 2019 Jul 9;6:228. doi: 10.3389/fvets.2019.00228. eCollection 2019.
, an intraerythrocytic protozoa, can cause an emerging tick-borne disease-Human babesiosis. The parasite can successfully invade host red blood cells owing to the assistance of molecules expressed by babesia. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the housekeeping intracellular glycolytic enzyme, can also be expressed in the external of cells, where contributes to binding to several molecules such as plasminogen and actin. In the present study, we identified GAPDH (BmGAPDH) and generated the recombinant BmGAPDH (rBmGAPDH) via an expression system. Furthermore, we confirmed its catalytic dehydration activity . Moreover, we also demonstrated that rBmGAPDH could bind to human plasminogen and mouse α-actin. In addition, we demonstrated that rBmGAPDH could recognize anti- mouse serum. In conclusion, BmGAPDH is a multifunctional glycolytic enzyme, which can bind to host plasminogen and α-actin.
巴贝斯虫是一种红细胞内的原生动物，可引发一种新出现的蜱传播疾病——人类巴贝斯虫病。由于巴贝斯虫表达的分子的协助，该寄生虫能够成功侵入宿主红细胞。甘油醛-3-磷酸脱氢酶（GAPDH）是细胞内管家糖酵解酶，也可在细胞外表达，在细胞外它有助于与纤溶酶原和肌动蛋白等多种分子结合。在本研究中，我们鉴定了巴贝斯虫甘油醛-3-磷酸脱氢酶（BmGAPDH），并通过表达系统制备了重组BmGAPDH（rBmGAPDH）。此外，我们证实了其催化脱水活性。而且，我们还证明rBmGAPDH能与人纤溶酶原和小鼠α-肌动蛋白结合。另外，我们证明rBmGAPDH能识别抗小鼠血清。总之，BmGAPDH是一种多功能糖酵解酶，它能与宿主纤溶酶原和α-肌动蛋白结合。
