Stanley E R
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2969-73. doi: 10.1073/pnas.76.6.2969.
Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified 125I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine CSF preparations that contain this subclass of CSF complete for all of the CSF binding sites on anti-L cell CSF antibody. With the exception of mouse serum, which can contain inhibitors of the bioassay, there is complete correspondence between activities determined by RIA and those determined by bioassay. The RIA is slightly more sensitive than the bioassay, detecting approximately 0.3 fmol of purified L cell CSF. It also detect this subclass of CSF in chickens, rats, and humans. In the mouse, the subclass is distinguished from other CSFs by a murine cell bioassay dose-response curve in which 90% of the response occurs over a 10-fold (rather than a 100-fold) increase in concentration, by stimulating the formation of colonies containing a high proportion of mononuclear (rather than granulocytic) cells, and by certain physical characteristics.
集落刺激因子（CSFs）可刺激未成熟前体细胞分化为成熟的粒细胞和巨噬细胞。纯化的125I标记的小鼠L细胞CSF已被用于开发一种放射免疫测定法（RIA），该方法可检测刺激巨噬细胞产生的CSF亚类。含有这种CSF亚类的小鼠CSF制剂可与抗L细胞CSF抗体上所有的CSF结合位点结合。除了可能含有生物测定抑制剂的小鼠血清外，RIA测定的活性与生物测定确定的活性完全一致。RIA比生物测定法稍敏感，可检测到约0.3 fmol的纯化L细胞CSF。它还能检测鸡、大鼠和人类中的这种CSF亚类。在小鼠中，该亚类与其他CSF的区别在于，其小鼠细胞生物测定剂量反应曲线中，90%的反应发生在浓度增加10倍（而非100倍）时，通过刺激形成含有高比例单核细胞（而非粒细胞）的集落，以及某些物理特性。
