Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, Texas 78229, USA.
Fertil Steril. 2012 May;97(5):1129-35.e1. doi: 10.1016/j.fertnstert.2012.02.007. Epub 2012 Feb 24.
To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis.
In vivo and vitro study.
University-based academic medical center.
PATIENT(S): Reproductive-age women undergoing surgery for benign conditions.
INTERVENTION(S): Peritoneal and endometrial tissue samples were obtained.
MAIN OUTCOME MEASURE(S): CSF-1 and C-FMS expression.
RESULT(S): Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis.
CONCLUSION(S): Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.
研究集落刺激因子 1（CSF-1）及其受体 C-FMS 在子宫内膜异位症中的表达和调节。
体内和体外研究。
大学附属学术医疗中心。
因良性疾病接受手术的育龄妇女。
获取腹膜和子宫内膜组织样本。
CSF-1 和 C-FMS 的表达。
与对照组相比，子宫内膜异位症患者的腹腔液中 CSF-1 水平显著升高。异位子宫内膜组织中 CSF-1 和 C-FMS 的表达分别增加了 3.5 倍和 1.7 倍，与在位组织相比。将子宫内膜细胞（来自已建立的细胞系或患者样本）与腹膜间皮细胞（PMCs）共培养导致 CSF-1 和 C-FMS 的表达增加。与没有子宫内膜异位症的患者相比，子宫内膜异位症患者的子宫内膜上皮细胞共培养中 C-FMS 和 CSF-1 的水平升高，但无统计学意义。
CSF-1 水平的升高可能有助于子宫内膜异位症病变的形成和进展。子宫内膜细胞与 PMCs 共培养后 CSF-1 水平升高表明，子宫内膜组织可能是腹膜 CSF-1 的来源。与 PMCs 共培养的子宫内膜异位症患者的子宫内膜细胞中 C-FMS 表达增加表明，参与病变形成的子宫内膜组织对 CSF-1 信号高度敏感。
