基于 WO 纳米片的过氧化物酶样活性的尿液中黄嘌呤的比色测定。

Colorimetric determination of xanthine in urine based on peroxidase-like activity of WO nanosheets.

机构信息

Ministry of Education Key Laboratory of Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China.

Clinical Laboratory, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

出版信息

Talanta. 2019 Nov 1;204:278-284. doi: 10.1016/j.talanta.2019.06.003. Epub 2019 Jun 3.

DOI:10.1016/j.talanta.2019.06.003

PMID:31357294

Abstract

Two-dimensional WO nanosheets were prepared by ultrasonic exfoliation of bulk WO·2HO in water and characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman, dynamic light scattering. The nanosheets were discovered to possess the peroxidase-like catalytic activity, which can catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine by HO. The catalytic mechanism was also investigated by the scavenger experiments. Taking advantage of the peroxidase-like activity of WO nanosheets, a facile colorimetric method for xanthine was developed by combining the oxidation reaction of xanthine catalyzed by xanthine oxidase. The linear range for xanthine was ranged from 25 to 200 μmol L. The limit of detection for xanthine was 1.24 μmol L. The colorimetric method was applied to determine xanthine in urine samples.

摘要

二维 WO 纳米片通过 bulk WO·2HO 在水中的超声剥离制备，并通过透射电子显微镜、原子力显微镜、X 射线衍射、X 射线光电子能谱、拉曼、动态光散射进行了表征。发现纳米片具有过氧化物酶样催化活性，可催化 HO 对 3,3',5,5'-四甲基联苯胺的氧化。还通过清除实验研究了催化机制。利用 WO 纳米片的过氧化物酶样活性，通过结合黄嘌呤氧化酶催化的黄嘌呤氧化反应，开发了一种简单的黄嘌呤比色法。黄嘌呤的线性范围为 25 至 200 μmol·L。黄嘌呤的检测限为 1.24 μmol·L。该比色法用于测定尿样中的黄嘌呤。