Department of Chemistry "Ugo Schiff", University of Florence, Via Della Lastruccia 3, 50019, Sesto Fiorentino (Fi), Italy; Analytical Chemistry Department, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Pasteur 4, Cluj-Napoca, Romania.
Department of Chemistry "Ugo Schiff", University of Florence, Via Della Lastruccia 3, 50019, Sesto Fiorentino (Fi), Italy.
Talanta. 2019 Nov 1;204:525-532. doi: 10.1016/j.talanta.2019.06.041. Epub 2019 Jun 12.
In this work, we present a smartphone-based multiplexed enzymatic biosensor utilizing the unique colorimetric properties of the poly(aniline-co-anthranilic acid) (ANI-co-AA) composite film coupled with horseradish peroxidase (HRP), glucose oxidase (GOx), horseradish peroxidase-glucose oxidase (GOx-HRP) and tyrosinase (Tyr) enzymes. The enzymes are immobilized on the composite polymer film by adsorption and they catalyze a reversible redox color change of the host polymer from green to blue in the presence of their substrate. A smartphone was applied as color detector, for image acquisition and data handling. A ColorLab android application, free of charge software application, was used to enable easy and clear display of the sensors' response indicating remarkable changes in the optical features. The results were confirmed by the spectrophotometric measurements. The developed colorimetric enzymatic biosensors were studied and optimized in relation to different experimental parameters. Moreover, the colorimetric enzymatic biosensors were applied to food and pharmaceutical analysis. It has been shown by these studies that the colorimetric biosensors are promising as quick and simple tests for handheld analysis in various fields.
在这项工作中，我们提出了一种基于智能手机的多重酶生物传感器，利用聚（苯胺-co-邻苯二甲酸酐）（ANI-co-AA）复合膜的独特比色特性，结合辣根过氧化物酶（HRP）、葡萄糖氧化酶（GOx）、辣根过氧化物酶-葡萄糖氧化酶（GOx-HRP）和酪氨酸酶（Tyr）酶。通过吸附，将酶固定在复合聚合物膜上，在其底物存在下，宿主聚合物发生可逆氧化还原颜色变化，从绿色变为蓝色。智能手机被用作颜色探测器，用于图像采集和数据处理。ColorLab 是一款免费的安卓应用程序，可用于轻松清晰地显示传感器的响应，表明光学特性发生了显著变化。结果通过分光光度法测量得到证实。对显色酶生物传感器进行了研究和优化，以考察不同的实验参数。此外，还将比色酶生物传感器应用于食品和药物分析。这些研究表明，比色生物传感器有望成为各种领域中用于手持式分析的快速简单测试方法。
