Khan Muhammad Jamshed, Kumari Suriya, Shameli Kamyar, Selamat Jinap, Sazili Awis Qurni
Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia.
Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
Materials (Basel). 2019 Jul 26;12(15):2382. doi: 10.3390/ma12152382.
Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was "face centered cubic (fcc)" as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.
纳米颗粒（NPs）经常被应用于多个领域。在过去十年中，银纳米颗粒（AgNPs）因其在极低用量下具有卓越的功效以及在较高温度下具有稳定性而吸引了研究人员。由于化学合成方法存在某些局限性，AgNPs可通过包括阳光、微波和紫外线（UV）辐射在内的物理方法获得。在当前研究中，通过波长为365nm的紫外线（UV）照射96小时实现了普鲁兰多糖介导的银纳米颗粒（P-AgNPs）的合成。紫外线照射24小时后形成了P-AgNPs，在紫外可见光谱中，96小时后其光谱最大值为415nm。粉末X射线衍射（PXRD）证实晶体结构为“面心立方（fcc）”。此外，高分辨率透射电子显微镜（HRTEM）证明P-AgNPs被一层薄薄的普鲁兰多糖覆盖，平均晶体尺寸为6.02±2.37。通过选区电子衍射（SAED）分析，纳米颗粒的平均晶格条纹间距被确认为0.235nm，具有准球形特征。这些绿色合成的P-AgNPs在掺入可食用薄膜后，可以作为一种有效的食品和肉类防腐剂加以利用。
