Hubei Collaborative Innovation Center for Grain Industry, Agricultural college, Yangtze University, Jingzhou 434025, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Int J Mol Sci. 2019 Jul 26;20(15):3674. doi: 10.3390/ijms20153674.
Nitrogen (N) is an essential nutrient for plants and a key limiting factor of crop production. However, excessive application of N fertilizers and the low nitrogen use efficiency (NUE) have brought in severe damage to the environment. Therefore, improving NUE is urgent and critical for the reductions of N fertilizer pollution and production cost. In the present study, we investigated the effects of N nutrition on the growth and yield of the two rice (Oryza sativa L.) cultivars, conventional rice Huanghuazhan and indica hybrid rice Quanliangyou 681, which were grown at three levels of N fertilizer (including 135, 180 and 225 kg/hm, labeled as N9, N12, N15, respectively). Then, a proteomic approach was employed in the roots of the two rice cultivars treated with N fertilizer at the level of N15. A total of 6728 proteins were identified, among which 6093 proteins were quantified, and 511 differentially expressed proteins were found in the two rice cultivars after N fertilizer treatment. These differentially expressed proteins were mainly involved in ammonium assimilation, amino acid metabolism, carbohydrate metabolism, lipid metabolism, signal transduction, energy production/regulation, material transport, and stress/defense response. Together, this study provides new insights into the regulatory mechanism of nitrogen fertilization in cereal crops.
氮（N）是植物的必需养分，也是作物生产的关键限制因素。然而，过量施用氮肥和低氮利用效率（NUE）已经对环境造成了严重破坏。因此，提高 NUE 对于减少氮肥污染和降低生产成本至关重要。在本研究中，我们研究了氮营养对两个水稻（Oryza sativa L.）品种生长和产量的影响，这两个品种分别是常规稻黄华占和籼型杂交稻荃两优 681，它们在三个氮肥水平（分别标记为 N9、N12 和 N15，包括 135、180 和 225 kg/hm）下生长。然后，我们采用蛋白质组学方法研究了氮肥水平为 N15 时两种水稻品种根系中的情况。共鉴定出 6728 种蛋白质，其中 6093 种蛋白质被定量，氮肥处理后两种水稻品种中发现了 511 种差异表达蛋白。这些差异表达蛋白主要参与氨同化、氨基酸代谢、碳水化合物代谢、脂质代谢、信号转导、能量产生/调节、物质运输以及应激/防御反应。总之，这项研究为禾本科作物氮素施肥的调控机制提供了新的见解。
