Biotechnology Group, TOTO Ltd. Research Institute, Chigasaki 253-8577, Japan.
Department of Urology, Graduate School of Medicine, Yokohama City University, Yokohama 236-0004, Japan.
Int J Mol Sci. 2019 Jul 28;20(15):3698. doi: 10.3390/ijms20153698.
Photodynamic diagnosis (PDD) can improve diagnostic accuracy by using PDD agents such as 5-aminolevulinic acid (ALA). However, the weakness and photobleaching of fluorescence of PDD agents may lead to insufficient fluorescence visibility for the detection of cancer during resection operations. We focused on the "fluorescence enhancement effect" resulting from the addition of polyethylene glycol-modified titanium dioxide nanoparticles (TiO-PEG NPs) to address these problems. The results showed that the combined administration of TiO-PEG NPs and ALA could enhance and prolong fluorescence in bladder cancer cells, similar to in the mixture alone. It was suggested that the fluorescence enhancement was related to the accumulation of TiO-PEG NPs in cells via endocytosis, causing the light scattering and enhancement of fluorescence. This fluorescence enhancement effect could be applicable for PDD.
光动力诊断（PDD）可以通过使用 5-氨基酮戊酸（ALA）等 PDD 试剂来提高诊断准确性。然而，PDD 试剂的荧光强度弱和光漂白性可能会导致在切除手术中对癌症的荧光检测不够明显。我们专注于通过添加聚乙二醇修饰的二氧化钛纳米粒子（TiO-PEG NPs）来解决这些问题的“荧光增强效果”。结果表明，TiO-PEG NPs 和 ALA 的联合给药可以增强和延长膀胱癌细胞的荧光，类似于单独混合的情况。这表明荧光增强与 TiO-PEG NPs 通过胞吞作用在细胞中的积累有关，导致光散射和荧光增强。这种荧光增强效果可应用于 PDD。
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