School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Anal Chim Acta. 2019 Oct 31;1078:119-124. doi: 10.1016/j.aca.2019.06.005. Epub 2019 Jun 5.
In this work, CaF nanoparticles were successfully synthesized by a simple direct precipitation method and firstly used as a peroxidase mimics for rapid and high sensitive colorimetric detection of aldosterone. The CaF nanoparticles were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM) and powder X-ray diffraction (XRD). The CaF nanoparticles can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue product oxidized TMB (oxTMB) in the presence of HO and this peroxidase-like activity of CaF is found out to follow Michaelis-Menten kinetics. Experiments showed that the catalytic mechanism of CaF nanoparticles was attributed to that it could result in the decomposition of HO to produce hydroxyl radicals (•OH). The absorbance change value of the reaction system was linear with the aldosterone concentration in the range of 2.0-40.0 nM, and the detection limit was 0.6 nM. Moreover, the developed method was applied to detect aldosterone in human serum samples. It provides a new platform for enzyme functional simulation and analytical sensing research.
在这项工作中，通过简单的直接沉淀法成功合成了 CaF 纳米颗粒，并首次将其用作过氧化物酶模拟物，用于快速、高灵敏比色检测醛固酮。通过扫描电子显微镜 (SEM)、透射电子显微镜 (TEM) 和粉末 X 射线衍射 (XRD) 对 CaF 纳米颗粒进行了表征。在 HO 的存在下，CaF 纳米颗粒可以氧化 3,3',5,5'-四甲基联苯胺 (TMB) 生成蓝色产物氧化 TMB (oxTMB)，并且发现 CaF 的这种过氧化物酶样活性遵循米氏动力学。实验表明，CaF 纳米颗粒的催化机制归因于它可以导致 HO 分解产生羟基自由基 (•OH)。反应体系的吸光度变化值与醛固酮浓度在 2.0-40.0 nM 范围内呈线性关系，检测限为 0.6 nM。此外，该方法还用于检测人血清样品中的醛固酮。它为酶功能模拟和分析传感研究提供了一个新的平台。
